Two Hybrid Technologies Methods and Protocols 2012th Edition by Bernhard Suter, Erich Wanker ISBN 1617794546 9781617794544

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ISBN 10: 1617794546 
ISBN 13: 9781617794544
Author: Bernhard Suter, Erich Wanker

Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Two Hybrid Technologies Methods and Protocols 2012th Table of contents:

Section I: High-Throughput Yeast Two-Hybrid Screening

This section focuses on various strategies and systems employed in yeast two-hybrid (Y2H) screening:

Matrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems
Array-Based Yeast Two-Hybrid Screens: A Practical Guide
High Throughput Yeast Two-Hybrid Screening
A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein-Protein Interaction Discovery
High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries
Section II: Innovative PPI Detection Methods

This section explores alternative high-throughput methodologies for PPI detection:

Virus-Human Cell Interactomes
Interactome Mapping in Malaria Parasites: Challenges and Opportunities
Mapping Interactomes with High Coverage and Efficiency Using the Shifted Transversal Design
Assigning Confidence Scores for Protein-Protein Interactions
The Integration and Annotation of the Human Interactome in the UniHI Database
Gene-Centered Yeast One-Hybrid Assays
One- plus Two-Hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein-Protein Interactions
Investigation of Membrane Protein Interactions Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System
Application of the Split-Protein Sensor Trp1 to Protein Interaction Discovery in the Yeast Saccharomyces cerevisiae
Tetracycline Repressor-Based Mammalian Two-Hybrid Systems
The Fluorescent Two-Hybrid (F2H) Assay for Direct Analysis of Protein-Protein Interactions in Living Cells
ArrayMAPPIT: a Screening Platform for Human Protein Interactome Analysis
MAPPIT as a High-Throughput Screening Assay for Modulators of Protein-Protein Interactions in HIV and HCV
Integrated Measurement of Split TEV and cis-Regulatory Assays Using EXT Encoded Reporter Libraries

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Bernhard Suter,Erich Wanker,Hybrid Technologies