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Transgenic Wheat Barley and Oats Production and Characterization Protocols 1st Edition by Paul A Lazzeri, Huw D Jones, Peter R Shewry ISBN 159745379X 9781597453790

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Transgenic Wheat Barley and Oats Production and Characterization Protocols 1st Edition Paul A. Lazzeri* Digital Instant Download

Author(s): Paul A. Lazzeri*, Huw D. Jones (auth.), Huw D. Jones, Peter R. Shewry (eds.)
ISBN(s): 9781597453790, 159745379X
Edition: 1
File Details: PDF, 5.18 MB
Year: 2009
Language: english
SKU: EB-1312838 Category: Tags: , , ,

Transgenic Wheat Barley and Oats Production and Characterization Protocols 1st Edition by Paul A Lazzeri, Huw D Jones, Peter R Shewry – Ebook PDF Instant Download/Delivery: 159745379X, 9781597453790
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ISBN 10: 159745379X 
ISBN 13: 9781597453790
Author: Paul A Lazzeri, Huw D Jones, Peter R Shewry

Understanding the physical and genetic structure of cereal genomes and how defined coding and non-coding regions interact with the environment to determine a phenotype are key to the future of plant breeding and agriculture. The production and characteri- tion of transgenic plants is a powerful reverse genetic strategy increasingly used in cereals research to ascribe function to defined DNA sequences. However, the techniques and resources required to conduct these investigations have, until recently, been difficult to achieve or totally lacking in wheat, barley and oat. This book brings together the l- est protocols for the transformation, regeneration and selection using both biolistic and Agrobacterium tumefaciens appropriate for these three species. It includes two chapters describing in vitro Agrobacterium co-cultivation, one leading to germ line transformation with no need for tissue culture-based regeneration. In addition, it has several chapters dedicated to the manipulation of gene expression and characterisation of the recombinant locus and transgenic plants. Finally, it tackles the issues of GM risk assessment, field trials and substantial equivalence in terms of transcriptomics, proteomics and metabolomics. Although this book is dedicated to the temperate small grain cereals wheat, barley and oats, many of the techniques described could be readily adapted for other cereals or plants generally. We thank all the contributing authors for their timely and informative chapters, the staff of Humana Press, especially John Walker for their guidance, and Helen Jenkins for her proof-reading, word processing and administrative support. v Contents.

Transgenic Wheat Barley and Oats Production and Characterization Protocols 1st Table of contents:

Biolistics Transformation of Wheat
Caroline A. Sparks, Huw D. Jones*
Pages 71-92
Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos
Huixia Wu, Angela Doherty, Huw D. Jones
Pages 93-103
Floral Transformation of Wheat
Sujata Agarwal, Star Loar, Camille Steber, Janice Zale*
Pages 105-113
Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation
Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul*, Tina Barsby
Pages 115-124
Barley Transformation Using Biolistic Techniques
Wendy A. Harwood*, Mark A. Smedley
Pages 125-136
Barley Transformation Using Agrobacterium-Mediated Techniques
Wendy A. Harwood*, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry, Mark A. Smedley, Nicola Leyl et al.
Pages 137-147
Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance
Shahina B. Maqbool*, Heng Zhong, Hesham F. Oraby, Mariam B. Sticklen
Pages 149-168
Gene and Protein Expression
Front Matter
Pages 170-170
Download chapter PDF 
Promoter Sequences for Defining Transgene Expression
Huw D. Jones*, Caroline A. Sparks
Pages 171-184
Down-Regulation of Gene Expression by RNA-Induced Gene Silencing
Silvia Travella*, Beat Keller
Pages 185-199
Characterisation of Transgenic Plants
Front Matter
Pages 202-202
Download chapter PDF 
Gene Insertion Patterns and Sites
Philippe Vain, Vera Thole
Pages 203-226
Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes
Trude Schwarzacher
Pages 227-246
Establishing Substantial Equivalence: Transcriptomics
María Marcela Baudo*, Stephen J. Powers, Rowan A. C. Mitchell, Peter R. Shewry
Pages 247-272

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