Transgenesis Techniques Principles and Protocols 3rd Edition by Leonie Ringrose, Elizabeth J Cartwright ISBN 1603270191 9781603270199
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ISBN 10: 1603270191
ISBN 13: 9781603270199
Author: Leonie Ringrose, Elizabeth J Cartwright
One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.
Transgenesis Techniques Principles and Protocols 3rd Table of contents:
Transgenesis in Drosophila melanogaster
Leonie Ringrose
Pages 3-19
Transgenesis in Caenorhabditis elegans
Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, Nektarios Tavernarakis
Pages 21-39
Transgenesis in Zebrafish with the Tol2 Transposon System
Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, Koichi Kawakami
Pages 41-63
Generation of Transgenic Frogs
Jana Loeber, Fong Cheng Pan, Tomas Pieler
Pages 65-72
Pronuclear DNA Injection for the Production of Transgenic Rats
Jean Cozzi, Ignacio Anegon, Valérie Braun, Anne-Catherine Gross, Christel Merrouche, Yacine Cherifi
Pages 73-88
Transgenesis in the Mouse
Front Matter
Pages 90-90
Download chapter PDF
Cell-Type-Specific Transgenesis in the Mouse
James Gulick, Jeffrey Robbins
Pages 91-104
Transgene Design and Delivery into the Mouse Genome: Keys to Success
Lydia Teboul
Pages 105-110
Overexpression Transgenesis in Mouse: Pronuclear Injection
Wendy J.K. Gardiner, Lydia Teboul
Pages 111-126
Gene-Targeting Vectors
J. Simon, C. Arthur, Victoria A. McGuire
Pages 127-144
Gene Trap: Knockout on the Fast Lane
Melanie Ullrich, Kai Schuh
Pages 145-159
Culture of Murine Embryonic Stem Cells
Ivana Barbaric, T. Neil Dear
Pages 161-184
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Tags: Leonie Ringrose, Elizabeth J Cartwright, Transgenesis Techniques, Protocols