Telomeres and Telomerase Methods and Protocols 1st Edition by Michael C Bibby, John A Double, Michael J Thompson ISBN 089603657X 9780896036574

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ISBN 10: 089603657X 
ISBN 13: 9780896036574
Author: Michael C Bibby, John A Double, Michael J Thompson

New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems.  For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli.  Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Telomeres and Telomerase Methods and Protocols 1st Table of contents:

Analysis of Average Telomere Length in Cultured Human Cells
Dan Liu
Pages 13-19
Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH)
Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
Pages 21-31
Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)
Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
Pages 33-46
Telomere G-Overhang Length Measurement Method 1: The DSN Method
Yong Zhao, Jerry W. Shay, Woodring E. Wright
Pages 47-54
Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA
Hidetoshi Tahara
Pages 55-61
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Yong Zhao, Jerry W. Shay, Woodring E. Wright
Pages 63-75
G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
Raveendra I. Mathad, Danzhou Yang
Pages 77-96
Analysis of Yeast Telomerase by Primer Extension Assays
Min Hsu, Neal F. Lue
Pages 97-106
Telomeric Repeat Amplification Protocol: Measuring the Activity of the Telomerase
Huawei Xin
Pages 107-111
CO-FISH, COD-FISH, ReD-FISH, SKY-FISH
Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin, Susan M. Bailey
Pages 113-124
Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques
Eladio Abreu, Rebecca M. Terns, Michael P. Terns
Pages 125-137
Cytogenetic Analysis of Telomere Dysfunction
Asha S. Multani, Sandy Chang
Pages 139-143
Probing the Telomere Damage Response
Rekha Rai, Sandy Chang
Pages 145-150
Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Wenbin Ma
Pages 151-159
Studying of Telomeric Protein–Protein Interactions by Bi-Molecular Fluorescence Complementation (BiFC) and Peptide Array-Based Assays
Wenbin Ma, Hyeung Kim, Zhou Songyang
Pages 161-171
Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation
Feng Wang, Ming Lei
Pages 173-187

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Tags: Michael C Bibby, John A Double, Michael J Thompson, Telomeres, Protocols