Single Molecule Tools Super Resolution Particle Tracking Multiparameter and Force Based Methods 1st Edition by Nils G Walter ISBN 0123814820 9780123814821

Single Molecule Tools Super Resolution Particle Tracking Multiparameter and Force Based Methods 1st Edition by Nils G Walter – Ebook PDF Instant Download/Delivery: 0123814820, 9780123814821
Full download Single Molecule Tools Super Resolution Particle Tracking Multiparameter and Force Based Methods 1st Edition after payment

Product details:

ISBN 10: 0123814820 
ISBN 13: 9780123814821
Author: Nils G Walter

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and “just look at the thing” as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

 Single Molecule Tools Super Resolution Particle Tracking Multiparameter and Force Based Methods 1st Table of contents:

Chapter 1: Super-Accuracy and Super-Resolution: GettingAround the Diffraction Limit
1. Overview: Accuracy and Resolution
2. Getting Super-Accuracy
3. Calculating Super-Accuracy
4. Reaching Super-Resolution
5. Future Directions
References
Chapter 2: Molecules and Methods for Super-Resolution Imaging
1. Introduction
2. Molecules for Super-Resolution Imaging
3. Selected Methods for Super-Resolution Imaging
Acknowledgments
References
Chapter 3: Tracking Single Proteins in Live Cells Using Single-Chain Antibody Fragment-Fluorescent Q
1. Introduction
2. The Method: Targeting QDs via a Single-Chain Variable Fragment-Hapten Pair
3. Functionalization of QDs
4. Quantification of the Number of FL Molecules per FL-pc-QD
5. Binding of FL-QDs to Anti-scFv Fusion Constructs
6. DNA Constructs for Single FL-QD Imaging in Live Cells
7. Single-Molecule Imaging of Live Mammalian Cells
Acknowledgments
References
Chapter 4: Recording Single Motor Proteins in the Cytoplasm of Mammalian Cells
1. Introduction
2. Basic Principles
3. Labeling Molecular Motors for In Vivo Observations
4. Instrumentation for Tracking Single Motors In Vivo
5. Detailed Experimental Procedures
6. Summary and Conclusions
Acknowledgments
References
Chapter 5: Single-Particle Tracking Photoactivated Localization Microscopy for Mapping Single-Molecu
1. Introduction
2. Description of the sptPALM Method
3. Labeling with Photoactivatable Fluorescent Probes
4. Tracking Single Molecules
5. Experimental Example: sptPALM on a Membrane Protein
6. Conclusions
References
Chapter 6: A Bird’s Eye View: Tracking SlowNanometer-Scale Movements ofSingle Molecular Nano-assembl
1. Introduction
2. DNA-Based Nanowalkers
3. Considerations for Fluorescence Imaging of Slowly Moving Particles
4. Single-Molecule Fluorescence Tracking of Nanowalkers
5. Extracting Super-Resolution Position Information
6. Concluding Remarks
Acknowledgments
References
Chapter 7: Anti-Brownian Traps for Studies on Single Molecules
1. Theoretical Overview
2. Anti-Brownian Trapping Systems
3. The ABEL Trap
4. Applications
5. Future Work: En Route to Single Fluorophores
Acknowledgments
References
Chapter 8: Plasmon Rulers as Dynamic Molecular Rulers in Enzymology
1. Introduction
2. The Basic Idea: Distance Dependence of Plasmon Coupling
3. Hardware Needed for Single Particle Rayleigh Scattering Spectroscopy
4. Which Readout-Intensity, Polarization, or Color?
5. Ruler Calibration?
6. Plasmon Ruler Assembly and Purification
7. Example 1: Dynamics of DNA Bending and Cleavage by Single EcoRV Restriction Enzymes
8. Example 2: Spermidine Modulated Ribonuclease Activity Probed by RNA Plasmon Rulers
9. Outlook
References
Chapter 9: Quantitative Analysis of DNA-Looping Kinetics from Tethered Particle Motion Experiments
1. Introduction
2. Change-Point Algorithm
3. Data Clustering and Expectation-Maximization Algorithm
4. Adaptation of the Method to the Case of TPM Data Analysis
5. Performance of the Method
6. Comparison with the Threshold Method
7. Application to TPM Experiments: CI-Induced Looping in lambda-DNA
8. Conclusions
Acknowledgments
References
Chapter 10: Methods in Statistical Kinetics
1. Introduction
2. The Formalism of Statistical Kinetics
3. Characterizing Fluctuations
4. Extracting Mechanistic Constraints from Moments
5. Conclusions and Future Outlook
Appendix
References
Chapter 11: Visualizing DNA Replication at the Single-Molecule Level
1. Introduction
2. Observing Replication Loops with Tethered Bead Motion
3. Fluorescence Visualization of DNA Replication
Acknowledgments
References
Chapter 12: Measurement of the Conformational State of F1-ATPase by Single-Molecule Rotation
1. Introduction
2. Sample Preparation
3. Single-Molecule Cross-Link Experiment
4. Pausing with AMP-PNP or/and N3-
Acknowledgments
References
Chapter 13: Magnetic Tweezers for the Study of DNA Tracking Motors
1. Introduction
2. Experimental Setup
3. Methods and Protocols
4. Application to the Study of FtsK
5. Application to the Study of the GP41 Helicase
6. Conclusions
Acknowledgments
References
Chapter 14: Single-Molecule Dual-Beam Optical Trap Analysis of Protein Structure and Function
1. Introduction
2. Insights into Myosin Function Using a Dual-Beam Optical Trap
3. Optical Trap Instrumentation
4. Optical Trapping Experiment
5. Data Analysis
6. Conclusion
Appendix
Acknowledgments
References
Chapter 15: An Optical Apparatus for Rotation and Trapping
1. Introduction
2. Optical Trapping and Rotation of Microparticles
3. The Instrument
4. Fabrication of Anisotropic Particles
5. Instrument Calibration
6. Simultaneous Application of Force and Torque Using Optical Tweezers
7. Conclusions
Acknowledgments
References
Chapter 16: Force-Fluorescence Spectroscopy at the Single-Molecule Level
1. Introduction
2. Setup
3. Optical Trapping
4. Fluorescence Detection
5. Coalignment of Confocal and Optical Trapping
6. Sample Preparation Protocols
7. Applications to Biological Systems
8. Outlook
Acknowledgments
References
Chapter 17: Combining Optical Tweezers, Single-Molecule Fluorescence Microscopy, and Microfluidics f
1. Introduction
2. Instrumentation
3. Preparation of Reagents
4. Combining Optical Trapping, Fluorescence Microscopy, and Microfluidics: Example Protocols
5. Conclusions
Acknowledgments
References
Chapter 18: Accurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection
1. Introduction
2. FRET Theory
3. Fluorescence Properties and Measurement Techniques
4. Qualitative Description of smFRET
5. Quantitative Description of smFRET
6. Discussion
Acknowledgments
References
Chapter 19: Atomic Force Microscopy Studies of Human Rhinovirus: Topologyand Molecular Forces
1. Introduction
2. Results and Discussion
References
Chapter 20: High-Speed Atomic Force Microscopy Techniques for Observing Dynamic Biomolecular Process
1. Introduction
2. Survey of Requirements for High-Speed Bio-AFM Imaging
3. Substrate Surfaces
4. Control of Diffusional Mobility
5. Protein 2D Crystals as Targets to Study
6. Low-Invasive Imaging
7. UV Flash-Photolysis of Caged Compounds
8. Cantilever Tip
References
Chapter 21: Nanopore Force Spectroscopy Tools for Analyzing Single Biomolecular Complexes
1. Introduction
2. The Nanopore Method
3. DNA Unzipping Kinetics Studied Using Nanopore Force Spectroscopy
4. Conclusions and Summary
Acknowledgments
References
Chapter 22: Analysis of Single Nucleic Acid Molecules with Protein Nanopores
1. Background: Analysis of Nucleic Acids with Nanopores
2. Electrical Recording with Planar Lipid Bilayers
3. Nanopores
4. Materials
5. Data Acquisition and Analysis

People also search for Single Molecule Tools Super Resolution Particle Tracking Multiparameter and Force Based Methods 1st:

    
single molecule super resolution microscopy
    
single molecule imaging
    
single molecule microscopy
    
single molecule techniques
    
single molecule localization microscopy

Tags: Nils G Walter, Single Molecule, Multiparameter, Force Based Methods