Single Cell Analysis Methods and Protocols 1st Edition by Dino Di Carlo, Henry Tat Kwong Tse, Daniel R Gossett, Sara Lindstrom, Helene Andersson Svahn ISBN 1617795674 9781617795671

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ISBN 10: 1617795674 
ISBN 13: 9781617795671
Author: Dino Di Carlo, Henry Tat Kwong Tse, Daniel R Gossett, Sara Lindstrom, Helene Andersson Svahn

This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Single Cell Analysis Methods and Protocols 1st Table of contents:

Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrinder
Prama Pallavi, Stefan Scheuermann
Pages 1-9
Circulating Tumor Cell Enrichment and Single-Cell Isolation Combining the CellSearch® and DEPArray™ Systems
Cäcilia Köstler, Bernhard Polzer, Barbara Alberter
Pages 11-42
Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients
Justyna Topa, Anna J. Żaczek, Aleksandra Markiewicz
Pages 43-52
Single-Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis-Initiating Cells
Pablo Hurtado, Inés Martínez-Pena, Roberto Piñeiro
Pages 53-63
Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System
Thais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G. J. Tibbe et al.
Pages 65-70
Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell
Miodrag Gužvić
Pages 71-100
Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients
Carolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer et al.
Pages 101-117
Establishing Single-Cell Clones from In Vitro-Cultured Circulating Tumor Cells
Teng Teng, Min Yu
Pages 119-126
Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules
Jakub Kochan, Mateusz Wawro
Pages 127-141
Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms Using Proximity Ligation Assay for RNA
Andrew D. Duckworth, Joseph R. Slupsky, Nagesh Kalakonda
Pages 143-165
Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells
Giancarlo Feliciello, Zbigniew Tadeusz Czyz, Bernhard M. Polzer
Pages 167-187
Single Cell Micro RNA Sequencing Library Preparation
Sarah M. Hücker, Stefan Kirsch
Pages 189-199
Immunoblot Analysis from Single Cells Using Milo™ Single-Cell Western Platform
Prashant V. Thakkar
Pages 201-214
Imaging of Subcellular Distribution of Platinum in Single Cells Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry
Amy J. Managh, Calum J. Greenhalgh
Pages 215-226
Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression
Cathryn R. Cadwell, Andreas S. Tolias
Pages 227-243
Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts
Michail Yekelchyk, Xiang Li, Stefan Guenther, Thomas Braun
Pages 245-257

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Tags: Dino Di Carlo, Henry Tat Kwong Tse, Daniel R Gossett, Sara Lindstrom, Helene Andersson Svahn, Cell Analysis, Protocols