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Recombinant proteins from plants methods and protocols 1st Edition by Christophe Sourrouille, Brian Marshall, David Lienard, Loic Faye, Veronique Gomord ISBN 1597454079 9781597454070

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Recombinant proteins from plants methods and protocols 1st Edition Christophe Sourrouille Digital Instant Download

Author(s): Christophe Sourrouille, Brian Marshall, David Liénard (auth.), Loïc Faye, Véronique Gomord (eds.)
ISBN(s): 9781597454070, 1597454079
Edition: 1
File Details: PDF, 12.42 MB
Year: 2009
Language: english
SKU: EB-1222348 Category: Tags: , , , ,

Recombinant proteins from plants methods and protocols 1st Edition by Christophe Sourrouille, Brian Marshall, David Lienard, Loic Faye, Veronique Gomord – Ebook PDF Instant Download/Delivery: 1597454079, 9781597454070
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ISBN 10: 1597454079 
ISBN 13: 9781597454070
Author: Christophe Sourrouille, Brian Marshall, David Lienard, Loic Faye, Veronique Gomord

Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state– the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.

Recombinant proteins from plants methods and protocols 1st Table of contents:

  1. From Neanderthal to Nanobiotech: from plant potions to pharming with plant factories

  2. Cowpea Mosaic Virus‑based systems for the expression of antigens and antibodies in plants

  3. Transient expression of antibodies in plants using syringe agroinfiltration

  4. Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants

  5. Production and localization of recombinant pharmaceuticals in transgenic seeds

  6. Production of antibody fragments in Arabidopsis seeds

  7. Production of plantibodies in Nicotiana plants

  8. Physcomitrella patens: a non‑vascular plant for recombinant protein production

  9. Production of recombinant proteins in suspension‑cultured plant cells

  10. Chloroplast-derived vaccine antigens and biopharmaceuticals: protocols for expression, purification or oral delivery and functional evaluation

  11. Protein body induction: a new tool to produce and recover recombinant proteins in plants

  12. A case study for plant-made pharmaceuticals comparing different plant expression and production systems

  13. Glycosylation of antibody therapeutics: optimisation for purpose

  14. N‑Glycosylation of Plant Recombinant Pharmaceuticals

  15. Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts

  16. Strategies for improving vaccine antigens expression in transgenic plants: fusion to carrier sequences

  17. Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies

  18. On‑chip detection of low‑molecular‑weight recombinant proteins in plant crude extracts by SELDI‑TOF MS

  19. Assessing the risk of undesirable immunogenicity/allergenicity of plant-derived therapeutic proteins

  20. Biosafety, risk assessment and regulation of plant-made pharmaceuticals

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