Pulse Field Gel Electrophoresis Methods and Protocols 1st Edition by Kieran Jordan, Marion Dalmasso ISBN 1493925989 9781493925988
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ISBN 10: 1493925989
ISBN 13: 9781493925988
Author: Kieran Jordan, Marion Dalmasso
This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Pulse Field Gel Electrophoresis Methods and Protocols 1st Table of contents:
Pulsed-Field Gel Electrophoresis for Disease Monitoring and Control
John Besser
Pages 3-7
Harmonization of PFGE Profile Analysis by Using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network
Benjamin Félix, Sophie Roussel, Bruno Pot
Pages 9-28
PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies
Marion Dalmasso, Kieran Jordan
Pages 29-34
Case Study of the Use of Pulsed Field Gel Electrophoresis in the Detection of a Food-Borne Outbreak
Niall De Lappe, Martin Cormican
Pages 35-40
PFGE and Pathogenic Bacteria
Front Matter
Pages 41-41
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Pulsed-Field Gel Electrophoresis for Listeria monocytogenes
Laura Luque-Sastre, Séamus Fanning, Edward M. Fox
Pages 43-53
Pulsed-Field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species
Qiongqiong Yan, Séamus Fanning
Pages 55-69
Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains
Paul Drean, Edward M. Fox
Pages 71-83
Pulsed-Field Gel Electrophoresis of Staphylococcus aureus
George R. Golding, Jennifer Campbell, Dave Spreitzer, Linda Chui
Pages 85-93
The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile
Wondwossen A. Gebreyes, Pamela R. F. Adkins
Pages 95-101
Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis
Carolina Lúquez, Lavin A. Joseph, Susan E. Maslanka
Pages 103-113
Pulsed-Field Gel Electrophoresis of Yersinia pestis
Tamara Revazishvili, Judith A. Johnson
Pages 115-128
Pulsed Field Gel Electrophoresis of Group A Streptococci
Luca Agostino Vitali, Giovanni Gherardi, Dezemona Petrelli
Pages 129-138
Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni
Ping Zhou, Omar A. Oyarzabal
Pages 139-156
Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa
Honghua Hu, Jim Manos
Pages 157-170
PFGE for Shiga Toxin-Producing Escherichia coli O157:H7 (STEC O157) and Non-O157 STEC
Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, Efrain M. Ribot
Pages 171-189
Pulsed-Field Gel Electrophoresis of Salmonella enterica
Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, Nicola Pugliese
Pages 191-210
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Kieran Jordan,Marion Dalmasso,Electrophoresis,Protocols