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Protoplast Technology Methods and Protocols 1st Edition by Kan Wang, Feng Zhang ISBN 1071621661 9781071621660

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Protoplast Technology Methods and Protocols 1st Edition Kan Wang Digital Instant Download

Author(s): Kan Wang, Feng Zhang
ISBN(s): 9781071621639, 1071621637
Edition: 1
File Details: PDF, 7.55 MB
Year: 2022
Language: english
SKU: EB-40667170 Category: Tags: ,

Protoplast Technology Methods and Protocols 1st Edition by Kan Wang, Feng Zhang – Ebook PDF Instant Download/Delivery: 1071621661, 978-1071621660
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Product details:

ISBN 10:    1071621661
ISBN 13: 978-1071621660
Author:     Kan Wang, Feng Zhang

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

Protoplast Technology Methods and Protocols 1st  Table of contents:

  1. Protoplast Isolation and Transfection in Physcomitrium patens
  2. Protoplast Isolation and Transfection in Bienertia sinuspersici
  3. Gene Functional Analysis in Arabidopsis using Protoplasts
  4. CRISPR/Cas9 Mutagenesis in Nicotiana tabacum
  5. Genome Editing in Nicotiana benthamiana via Protoplast Transformation
  6. Protoplast Transfection in Rice, Maize, and Wheat
  7. Protoplast Studies in Soybean, Camelina, and Oilseed Crops
  8. High-Throughput Transfection and Fluorescence-Activated Sorting for Gene Editing in Soybean
  9. Transcription Activator Characterization in Setaria viridis

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