Protocols in In Vitro Hepatocyte Research 1st Edition by Mathieu Vinken, Vera Rogiers ISBN 1493920731 9781493920730
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ISBN 10: 1493920731
ISBN 13: 9781493920730
Author: Mathieu Vinken, Vera Rogiers
This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.
Protocols in In Vitro Hepatocyte Research 1st Table of contents:
Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments
Lyndsey Houseman, Mina Edwards, Ian R. Phillips, Elizabeth A. Shephard
Pages 3-12
Cryopreservation of Hepatocytes
Nicola J. Hewitt, Albert P. Li
Pages 13-26
Culture Conditions Promoting Hepatocyte Proliferation and Cell Cycle Synchronization
Anne Corlu, Pascal Loyer
Pages 27-51
Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, Mathieu Vinken
Pages 53-76
Culture and Functional Characterization of Human Hepatoma HepG2 Cells
María Teresa Donato, Laia Tolosa, María José Gómez-Lechón
Pages 77-93
Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
Mathieu Vinken, Michaël Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, Vera Rogiers
Pages 95-103
Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes
Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, David C. Hay
Pages 105-111
Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation
Joery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers
Pages 113-122
Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling
Richard L. Gieseck III, Ludovic Vallier, Nicholas R. F. Hannan
Pages 123-142
Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation
Varvara Gouliarmou, Olavi Pelkonen, Sandra Coecke
Pages 143-159
Coculture and Long-Term Maintenance of Hepatocytes
Merav Cohen, Gahl Levy, Yaakov Nahmias
Pages 161-173
Primary Hepatocytes in Sandwich Culture
Janneke Keemink, Marlies Oorts, Pieter Annaert
Pages 175-188
Establishing Liver Bioreactors for In Vitro Research
Sofia P. Rebelo, Rita Costa, Marcos F. Q. Sousa, Catarina Brito, Paula M. Alves
Pages 189-202
Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture
Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, Tamara Vanhaecke
Pages 203-211
Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors
Marta Benet, Ramiro Jover, Roque Bort
Pages 213-221
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Tags: Mathieu Vinken, Vera Rogiers, Protocols, Vitro Hepatocyte