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Protein Phosphatase Protocols 1st Edition by Shirish Shenolikar, Greg Moorhead ISBN 159745267X 9781597452670

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Protein Phosphatase Protocols 1st Edition Shirish Shenolikar (Auth.) Digital Instant Download

Author(s): Shirish Shenolikar (auth.), Greg Moorhead (eds.)
ISBN(s): 9781597452670, 159745267X
Edition: 1
File Details: PDF, 5.82 MB
Year: 2007
Language: english
SKU: EB-1313538 Category: Tags: ,

Protein Phosphatase Protocols 1st Edition by Shirish Shenolikar, Greg Moorhead – Ebook PDF Instant Download/Delivery: 159745267X, 9781597452670
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ISBN 10: 159745267X 
ISBN 13: 9781597452670
Author: Shirish Shenolikar, Greg Moorhead

Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics. Included protocols utilize several model organisms, including yeast, Drosophila, and various plant and human cells. Protein Phosphatase Protocols provides a concise introduction to protein phosphatase research, with particular attention to phosphatase families. Assays for identification, quantification, purification, and functional characterization are presented throughout in specific detail.

Protein Phosphatase Protocols 1st Table of contents:

Analysis of Protein Phosphatases
Shirish Shenolikar
Pages 1-8
A Brief Introduction to the Protein Phosphatase Families
Tomas Mustelin
Pages 9-22
Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases
Mark Swingle, Li Ni, Richard E. Honkanen
Pages 23-38
Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose
Greg B. G. Moorhead, Timothy A. J. Haystead, Carol MacKintosh
Pages 39-45
Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated
Aaron A. Goodarzi, Pauline Douglas, Greg B. G. Moorhead, Susan P. Lees-Miller
Pages 47-59
An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity
Adam M. Wegner, Jamie L. McConnell, Randy D. Blakely, Brian E. Wadzinski
Pages 61-69
An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species
Matthew S. Gentry, Richard L. Hallberg, David C. Pallas
Pages 71-83
Mutagenesis and Expression of the Scaffolding Aα and Aβ Subunits of PP2A
Ralf Ruediger, Jin Zhou, Gernot Walter
Pages 85-99
Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits
Deanna G. Adams, Brian E. Wadzinski
Pages 101-111
Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies
Gernot Walter, Jin Zhou, Ralf Ruediger
Pages 113-126
Purification of PP2Ac from Bovine Heart
Hue T. Tran, Tony S. Ferrar, Anne Ulke-Lemée, Greg B. G. Moorhead
Pages 127-132
Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions
Laura Trinkle-Mulcahy, Janet Chusainow, Yun Wah Lam, Sam Swift, Angus Lamond
Pages 133-154
Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins
Daimark Bennett, Luke Alphey
Pages 155-179
Identification of Cellular Protein Phosphatase-1 Regulators
David W. Roadcap, Matthew H. Brush, Shirish Shenolikar
Pages 181-196
Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2
Masumi Eto, David L. Brautigan
Pages 197-208
Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C
Michael P. Walsh, Marija Susnjar, Jingti Deng, Cindy Sutherland, Enikő Kiss, David P. Wilson
Pages 209-223
Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin
Meredith Borman, Justin MacDonald
Pages 225-233
Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry
Edmund P. Walsh, Douglas J. Lamont, Kenneth A. Beattie, Michael J. R. Stark
Pages 235-246
Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity
Nicole Bäumer, Anette Mäurer, Josef Krieglstein, Susanne Klumpp
Pages 247-260

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Tags: Shirish Shenolikar, Greg Moorhead, Protein Phosphatase