Protein Chromatography Methods and Protocols 2nd Edition by Dermot Walls, Sinead T Loughran ISBN 1493964100 9781493964109
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Product details:
ISBN 10: 1493964100
ISBN 13: 9781493964109
Author: Dermot Walls, Sinéad T. Loughran
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
Table of contents:
Overview Chapters
Front Matter
Chapter 1 A Synopsis of Proteins and Their Purification
Chapter 2 Gel-Filtration Chromatography
Chapter 3 Immunoaffinity Chromatography: Concepts and Applications
Chapter 4 Avoiding Proteolysis During Protein Purification
Chapter 5 Scale-Up of Protein Purification: Downstream Processing Issues
Chapter 6 Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources
Chapter 7 Protein Stability: Enhancement and Measurement
Chapter 8 Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Protocols Chapters
Front Matter
Chapter 9 Storage and Lyophilization of Pure Proteins
Chapter 10 Differential Precipitation and Solubilization of Proteins
Chapter 11 Ion-Exchange Chromatography: Basic Principles and Application
Chapter 12 Protein Quantitation and Analysis of Purity
Chapter 13 Purification of Proteins Fused to Maltose-Binding Protein
Chapter 14 Purification of Polyhistidine-Tagged Proteins
Chapter 15 Purification of Antibodies Using Affinity Chromatography
Chapter 16 Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
Chapter 17 Measuring Protein–Protein Interactions Using Biacore
Chapter 18 Hydrophobic Interaction Chromatography
Chapter 19 Fast Protein Liquid Chromatography
Chapter 20 Clinical Proteomics: Liquid Chromatography–Mass Spectrometry (LC–MS) Purification Systems
Chapter 21 Strategies for the Purification of Membrane Proteins
Chapter 22 Antimicrobial Peptide Production and Purification
Chapter 23 Lectin Affinity Chromatography (LAC)
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Tags: Dermot Walls, Sinead T Loughran, Protein Chromatography, Methods, Protocols