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Protein Arrays Methods and Protocols 1st Edition by Eric Fung ISBN 1592597599 9781592597598

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Protein Arrays Methods and Protocols 1st Edition Eric Fung Digital Instant Download

Author(s): Eric Fung
ISBN(s): 9781592597598, 1592597599
Edition: 1
File Details: PDF, 4.53 MB
Year: 2004
Language: english
SKU: EB-921878 Category: Tag:

Protein Arrays Methods and Protocols 1st Edition by Eric Fung – Ebook PDF Instant Download/Delivery: 1592597599, 9781592597598

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Product details:

ISBN 10: 1592597599

ISBN 13: 9781592597598 

Author: Eric Fung

Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.

Table of contents:

  1. Front Matter

  2. Protein Arrays From cDNA Expression Libraries

  3. Protein Expression Arrays For Proteomics

  4. Generation Of Protein In Situ Arrays By DiscernArray™ Technology

  5. Multiplexed Protein Analysis Using Spotted Antibody Microarrays

  6. Target-Assisted Iterative Screening Of Phage Surface Display cDNA Libraries

  7. Design, Construction, And Use Of Tissue Microarrays

  8. A Streptavidin-Biotin-Based Microarray Platform For Immunoassays

  9. Site-Specific Immobilization Of Biotinylated Proteins For Protein Microarray Analysis

  10. Processing Of Photoaptamer Microarrays

  11. Use Of A Small Molecule-Based Affinity System For The Preparation Of Protein Microarrays

  12. Evaluation Of Antibodies And Microarray Coatings As A Prerequisite For The Generation Of Optimized Antibody Microarrays

  13. Printing Functional Protein Microarrays Using Piezoelectric Capillaries

  14. The Use Of Precision Glass Syringes And A Noncontact Microsolenoid Dispenser For The Production Of High-Throughput Low-Density Arrays

  15. A Protein Microarray ELISA For Screening Biological Fluids

  16. Protein-Domain Microarrays

  17. SH3 Domain Protein-Binding Arrays

  18. Site-Specific Peptide Immobilization Strategies For The Rapid Detection Of Kinase Activity On Microarrays

  19. Simultaneous Monitoring Of Multiple Kinase Activities By SELDI-TOF Mass Spectrometry

  20. Cytokine Protein Arrays

  21. Fabrication And Application Of G Protein-Coupled Receptor Microarrays

  22. ProteinChip® Array-Based Amyloid β Assays

  23. Serum Protein-Expression Profiling Using The ProteinChip® Biomarker System

  24. Protein Arrays For Serodiagnosis Of Disease

  25. Back Matter

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Tags: Eric Fung, Protein, Arrays, Methods, Protocols