Proteases and Cancer Methods and Protocols Methods in Molecular Biology 1st Edition by Salvatore Santamaria ISBN 1071635883 9781071635889

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ISBN 10: 1071635883
ISBN 13: 9781071635889
Author: Salvatore Santamaria

This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography,  angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences.

Proteases and Cancer Methods and Protocols Methods in Molecular Biology 1st Table of contents:

Dissecting Degradomes: Analysis of Protease-Coding Genes
Ángel Álvarez-Eguiluz, Ander Díaz-Navarro, Xose S. Puente
Pages 1-13
Identification of Protease Cleavage Sites and Substrates in Cancer by Carboxy-TAILS (C-TAILS)
Nestor Solis, Christopher M. Overall
Pages 15-28
Identification of Proteolytic Cleavage Sites of EphA2 by Membrane Type 1 Matrix Metalloproteinase on the Surface of Cancer Cells
Keiji Kikuchi, Hiroko Kozuka-Hata, Masaaki Oyama, Motoharu Seiki, Naohiko Koshikawa
Pages 29-37
Biotin-Chasing Assay to Evaluate uPAR Stability and Cleavage on the Surface of Cells
Vladimir Leksa, Herbert B. Schiller, Hannes Stockinger
Pages 39-47
Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease
Pedro M. Quirós
Pages 49-56
A Simple Cell-Based Assay for the Detection of Surface Protein Shedding by Rhomboid Proteases
Angela Moncada-Pazos, Adam Graham Grieve
Pages 57-64
Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm
Dong Hyun Nam, Ki Baek Lee, Xin Ge
Pages 65-72
Autophagy and Proteases: Basic Study of the Autophagic Flux by Western Blot
Álvaro F. Fernández
Pages 73-81
Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture
Aastha Chhabra, Vibha Rani
Pages 83-96
Analysis of Enzymatic Activity of Matrix Metalloproteinase (MMP) by Collagen Zymography in Melanoma
Vijay Walia, Yardena Samuels
Pages 97-106
Measurement of Protease Activities Using Fluorogenic Substrates
Salvatore Santamaria, Hideaki Nagase
Pages 107-122
Targeting the Expression of Cathepsin B Using CRISPR/Cas9 System in Mammalian Cancer Cells
Manu Gnanamony, Christopher S. Gondi
Pages 123-131
Assessing the Influence of a Protease in Cell Migration Using the Barrier-Migration Assay
Tania Fontanil, Yamina Mohamedi, Santiago Cal, Álvaro J. Obaya
Pages 133-143
Analysis of Invasive Activity of CAF Spheroids into Three Dimensional (3D) Collagen Matrices
María Ángeles Villaronga, Saúl Álvarez Teijeiro, Francisco Hermida-Prado, Marta Garzón-Arango, Victoria Sanz-Moreno, Juana María García-Pedrero
Pages 145-154
Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains Using Tri-Dimensional (3D) Spheroid Invasion Assay
Erico Tosoni Costa, Anamaria Aranha Camargo
Pages 155-168
Analyzing the Type II Transmembrane Serine Protease Hepsin-Dependent Basement Membrane Remodeling in 3D Cell Culture
Shishir M. Pant, Denis Belitskin, Hanna Ala-Hongisto, Juha Klefström, Topi A. Tervonen
Pages 169-178
Evaluation of Tumor Vasculature Using a Syngeneic Tumor Model in Wild-Type and Genetically Modified Mice
Francisco Javier Rodríguez-Baena, Silvia Redondo-García, María del Carmen Plaza-Calonge, Rubén Fernández-Rodríguez, Juan Carlos Rodríguez-Manzaneque
Pages 179-192
3D Image Analysis of the Microvasculature in Healthy and Diseased Tissues
Álvaro Sahún-Español, Cristina Clemente, Alicia G. Arroyo
Pages 193-212
Human Tumor Tissue-Based 3D In Vitro Invasion Assays
Pirjo Åström, Ritva Heljasvaara, Pia Nyberg, Ahmed Al-Samadi, Tuula Salo
Pages 213-221

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Salvatore Santamaria,Cancer,Protocols Methods