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Programmed Cell Death Part A 1st Edition by Roya Khosravi Far, Zahra Zakeri, Richard A Lockshin, Mauro Piacentini ISBN 0123743125 9780123743121

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Programmed Cell Death Part A 1st Edition Roya Khosravi-Far Digital Instant Download

Author(s): Roya Khosravi-Far, Zahra Zakeri, Richard A. Lockshin, Mauro Piacentini
ISBN(s): 0123743125
Edition: 1
File Details: PDF, 13.14 MB
Year: 2008
Language: english
SKU: EB-2106652 Category: Tags: , , ,

Programmed Cell Death Part A 1st Edition by Roya Khosravi Far, Zahra Zakeri, Richard A Lockshin, Mauro Piacentini – Ebook PDF Instant Download/Delivery: 0123743125, 9780123743121
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ISBN 10: 0123743125
ISBN 13: 9780123743121
Author: Roya Khosravi Far, Zahra Zakeri, Richard A Lockshin, Mauro Piacentini

The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (UK),H. Robert Horvitz (US)and John E. Sulston (UK) “for their discoveries concerning genetic regulation of organ development and programmed cell death.” Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death.

*Provides the necessary, trusted methods to carry out this research on the latest techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies
*Presents key methods on studying tumors and how these cancer cells evade cell death
*Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over

Programmed Cell Death Part A 1st Table of contents:

Chapter 1: Analyzing Morphological and Ultrastructural Features in Cell Death

  1. Introduction

  2. The Contribution of Morphological Analyses

  3. Light Microscopy

  4. Sample Processing for Light Microscopy

  5. Electron Microscopy

  6. Scanning Electron Microscopy (SEM)
    References

Chapter 2: Evaluation of Some Cell Death Features by Real Time Real Space Microscopy

  1. Mitochondria and Cell Death

  2. Bax, Mitochondria, and Calcium Signaling

  3. Calcium Signaling and Neurodegenerative Processes
    Acknowledgments
    References

Chapter 3: Analysis of Apoptotic Pathways by Multiparametric Flow Cytometry: Application to HIV Infection

  1. Introduction

  2. Intracellular Expression of Bcl-2 Family Members

  3. Analysis of Mitochondrial Transmembrane Potential

  4. DNA Fragmentation Assays (ISNT and TUNEL)

  5. Loss of Plasma Membrane Asymmetry (Annexin-V Assay)

  6. Plasma Membrane Integrity (7-AAD Assay)

  7. Cell-Mediated Cytotoxicity Assay

  8. Conclusions
    Acknowledgments
    References

Chapter 4: Methods to Analyze the Palmitoylated CD95 High Molecular Weight DISC

  1. Introduction

  2. Sucrose Density Gradients

  3. Association of CD95hi with Active Caspase-8

  4. Role of Palmitoylation

  5. Conclusions
    Acknowledgments
    References

Chapter 5: Immunomagnetic Isolation of Tumor Necrosis Factor Receptosomes

  1. Introduction

  2. TNF-R1 Apoptosis Signaling

  3. Magnetic Labeling and Isolation

  4. Experimental Procedures

  5. Conclusions
    Acknowledgments
    References

Chapter 6: Analyzing Lipid Raft Dynamics during Cell Apoptosis

  1. Introduction

  2. Biochemical Approaches

  3. Morphological Analyses
    References

Chapter 7: Apoptosome Assembly

  1. Introduction

  2. Caspase-9 Activation

  3. Dronc Activation

  4. CED-3 Activation

  5. Conclusion
    Acknowledgments
    References

Chapter 8: Caspases: Determination of Their Activities in Apoptotic Cells

  1. Introduction

  2. Methods for Caspase Activity Analysis

  3. Conclusion
    Acknowledgments
    References

Chapter 9: Lysosomes in Apoptosis

  1. Introduction

  2. Lysosomal Membrane Permeabilization

  3. Detection Methods

  4. Acid Vesicle Staining

  5. LMP Validation

  6. Cytosolic Extract Preparation

  7. Cathepsins

  8. Synthetic Substrates

  9. Protein Substrate Cleavage
    References

Chapter 10: More Than Two Sides of a Coin? (TG2 Functions)

  1. Introduction

  2. TG2 Activity Assays

  3. GTPase Assay

  4. TG2 Knockout Mice

  5. TG2 Detection

  6. Phagocytosis Assays

  7. TG2 as Protein Disulfide Isomerase

  8. Conclusions
    Acknowledgments
    References

Chapter 11: Granzymes and Cell Death

  1. Introduction

  2. Perforin Purification

  3. Recombinant Granzyme B

  4. Recombinant Granzyme A

  5. Granzyme-Mediated Apoptosis Assay

  6. Conclusion
    Acknowledgments
    References

Chapter 12: The Proapoptotic Bcl-2 Member Bid at the Crossroads of DNA Damage Response

  1. Introduction

  2. Isolation of Myeloid Precursor Cells

  3. Lin- Cell Purification

  4. Sca-1 Selection

  5. Magnetic Separation

  6. Cell Culture

  7. WEHI-Conditioned Medium

  8. Chromosomal Breakage Assays

  9. DNA Synthesis Assay

  10. Data Analysis

  11. Subcellular Fractionation

  12. Immunofluorescence

  13. Annexin V Staining

  14. Conclusion
    Acknowledgments
    References

Chapter 13: ASC Pyroptosome: Assembly, Purification, and Activity Assay

  1. Introduction

  2. Methods

  3. ASC-GFP Fusion in THP-1

  4. Biochemical Assay of Pyroptosome Formation

  5. In Vitro Assembly from Cell Lysates

  6. Recombinant Pyroptosome Assembly
    Acknowledgments
    References

Chapter 14: Nucleases in Programmed Cell Death

  1. Introduction

  2. CAD Assays

  3. DNase II Assay

  4. Apoptotic DNA Degradation

  5. Macrophage DNA Degradation
    References

Chapter 15: Detection of Autophagy in Cell Death

  1. Introduction

  2. EM Identification of Autophagic Structures

  3. Lysosomal Enzyme Activity

  4. Fluorescent Molecule Uptake

  5. Autophagy-Related Genes

  6. Inhibition Assays
    References

Chapter 16: Distinguishing Apoptotic from Necrotic Cells and Their Clearance

  1. Introduction

  2. Intact Cell Analysis

  3. Biochemical Markers

  4. Cell-Cell Interactions
    Acknowledgments
    References

Chapter 17: 2D Gel-Based Analysis of the Demolition Phase of Apoptosis

  1. Introduction

  2. Cell-Free Extract

  3. 2D SDS-PAGE

  4. Protein ID via MALDI-TOF

  5. Conclusion
    Acknowledgments
    References

Chapter 18: Mitochondrial Membrane Permeabilization in Apoptosis

  1. Introduction

  2. Outer Membrane Permeabilization

  3. Inner Membrane Permeabilization

  4. Guidelines

  5. Notes
    Acknowledgments
    References

Chapter 19: Oxidative Lipidomics of Programmed Cell Death

  1. Introduction

  2. Materials and Methods

  3. Results and Discussion
    Acknowledgments
    References

Chapter 20: Endoplasmic Reticulum Stress-Induced Apoptosis In Vivo

  1. ER and UPR Overview

  2. ER Stress-Induced Apoptosis

  3. In Vivo Characterization Methods
    Acknowledgments
    References

Chapter 21: Organelle Intermixing and Membrane Scrambling in Cell Death

  1. Introduction

  2. Organelle Intermixing Methods

  3. Caspase-Dependent Traffic Changes

  4. Outlook
    Acknowledgments
    References

Chapter 22: Glucose Metabolism and Cell Death

  1. Introduction

  2. Methods

  3. Conclusion

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Tags: Roya Khosravi Far, Zahra Zakeri, Richard A Lockshin, Mauro Piacentini, Death