Polyadenylation Methods and Protocols 1st Edition by Joanna Rorbach, Agnieszka J Bobrowicz ISBN 1627039708 9781627039703

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ISBN 10: 1627039708 
ISBN 13: 9781627039703
Author: Joanna Rorbach, Agnieszka J Bobrowicz

In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Polyadenylation Methods and Protocols 1st Table of contents:

• Polyadenylation Machinery

  • Enzymes Involved in Polyadenylation
  • Mechanism of Poly(A) Tail Addition
  • Regulation of Polyadenylation

• Methods for Studying Polyadenylation

  • RNA Isolation and Purification for Polyadenylation Studies
  • Detection of Polyadenylation
  • Poly(A) RNA Selection and Enrichment

• Polyadenylation Assays

  • In Vitro Polyadenylation Assays
  • In Vivo Methods for Studying Polyadenylation
  • Northern Blot Analysis for Polyadenylation

• Poly(A) Tail Length Analysis

  • Techniques for Measuring Poly(A) Tail Length
  • Use of Primer Extension and PCR-Based Methods
  • High-Throughput Approaches to Poly(A) Length Determination

• Polyadenylation-Dependent RNA Stability

  • Role of Poly(A) Tail in RNA Stability
  • Methods to Assess mRNA Degradation Rates
  • Poly(A) Tail Shortening and its Biological Implications

• Manipulating Polyadenylation in Cells

  • Genetic Approaches to Modify Polyadenylation
  • RNA Editing and Polyadenylation Site Selection
  • Effects of Polyadenylation on Gene Expression

• Polyadenylation in Different Organisms

  • Polyadenylation in Mammals
  • Polyadenylation in Plants and Yeast
  • Comparative Analysis of Polyadenylation Mechanisms

• Advanced Techniques in Polyadenylation Research

  • Mass Spectrometry Approaches for Polyadenylation Studies
  • Single-Molecule Techniques for Poly(A) Analysis
  • RNA-Seq and Polyadenylation Profiling

• Polyadenylation and Disease

  • Aberrant Polyadenylation in Human Diseases
  • Role of Polyadenylation in Cancer and Neurodegenerative Diseases
  • Therapeutic Approaches Targeting Polyadenylation

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Joanna Rorbach,Agnieszka J Bobrowicz,Polyadenylation Methods,Protocols