Plant Protein Secretion Methods and Protocols 2nd edition by Liwen Jiang, Jinbo Shen, Caiji Gao, Xiangfeng Wang ISBN 1071640585 978-1071640586
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Product details:
ISBN 10: 1071640585
ISBN 13: 978-1071640586
Author: Liwen Jiang, Jinbo Shen, Caiji Gao, Xiangfeng Wang
This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.
Table of contents:
Conventional and Unconventional Protein Secretion in Yeast and Animal Cells
An Overview of Protein Secretion in Plant Cells
Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation
Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition
Proteomic Analysis of Secreted Vesicle Proteins from the Plant Cells
Extracellular Vesicle Isolation and Mass Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana
Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.
Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.
Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.
In Vitro Reconstitution of Plant COPII Vesicles.
Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.
Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.
Polar Localization
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Liwen Jiang,Jinbo Shen,Caiji Gao,Xiangfeng Wang,Plant Protein,Methods,Protocols