Plant Cold Acclimation Methods and Protocols 1st Edition by Dirk Hincha, Ellen Zuther ‎1493946153 978-1493946150

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Product details:

ISBN-10 ‏ : ‎ 1493946153

ISBN-13 ‏ : ‎ 978-1493946150

Author: Dirk K. Hincha, Ellen Zuther 

Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Plant Cold Acclimation Methods and Protocols 1st Table of contents:

Introduction: Plant Cold Acclimation and Winter Survival

Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays

Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements

Infrared Thermal Analysis of Plant Freezing Processes

Conducting Field Trials for Frost Tolerance Breeding in Cereals

A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes

Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

Identification of Arabidopsis Mutants with Altered Freezing Tolerance

Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Using Pixel-Based Microscope Images to Generate 3D Reconstructions of Frozen and Thawed Plant Tissue

Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography

Proteomic Approaches to Identify Proteins Responsive to Cold Stress

Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation

Subcellular Compartmentation of Metabolites Involved in Cold Acclimation

Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime

 

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Tags: Plant Cold, Acclimation, Methods, Protocols, Dirk Hincha, Ellen Zuther