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PCR Protocols 3rd Edition by Zaheer Khan, Daniel J Park ISBN 160761944X 9781607619444

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PCR Protocols 3rd Edition Zaheer Khan (Auth.) Digital Instant Download

Author(s): Zaheer Khan (auth.), Daniel J. Park (eds.)
ISBN(s): 9781607619444, 160761944X
Edition: 3
File Details: PDF, 33.31 MB
Year: 2011
Language: english
SKU: EB-1834782 Category: Tags: ,

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ISBN 10: 160761944X 
ISBN 13: 9781607619444
Author: Zaheer Khan, Daniel J Park

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.

PCR Protocols 3rd Table of contents:

Long-Range PCR with a DNA Polymerase Fusion
Holly H. Hogrefe, Michael C. Borns
Pages 17-23
Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures
Bin Yin
Pages 25-42
Lariat-Dependent Nested PCR for Flanking Sequence Determination
Daniel J. Park
Pages 43-55
CODEHOP PCR and CODEHOP PCR Primer Design
Jeannette P. Staheli, Richard Boyce, Dina Kovarik, Timothy M. Rose
Pages 57-73
Sequencing of Difficult DNA Regions by SAM Sequencing
Keith R. Mitchelson
Pages 75-88
Expression, Epigenetic and Copy Number Analysis
Front Matter
Pages 89-89
Download chapter PDF 
A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis
Kazuki Kurimoto, Mitinori Saitou
Pages 91-111
Quantitation of MicroRNAs by Real-Time RT-qPCR
Caifu Chen, Ruoying Tan, Linda Wong, Richard Fekete, Jason Halsey
Pages 113-134
High-Throughput SuperSAGE
Hideo Matsumura, Kentaro Yoshida, Shujun Luo, Detlev H. Krüger, Günter Kahl, Gary P. Schroth et al.
Pages 135-146
Deep Cap Analysis of Gene Expression
Junpei Kurosawa, Hiromi Nishiyori, Yoshihide Hayashizaki
Pages 147-163
Linking Emulsion PCR Haplotype Analysis
James G. Wetmur, Jia Chen
Pages 165-175
PAP-LMPCR: An Improved, Sequence-Selective Method for the In Vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure
Richard Ingram, Arthur Riggs, Constanze Bonifer
Pages 177-192
The Many Faces of MLPA
Thomas Ohnesorg, Erin Turbitt, Stefan J. White
Pages 193-205
Assessing Gene-Specific Methylation Using HRM-Based Analysis
Ee Ming Wong, Alexander Dobrovic
Pages 207-217

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Tags: Zaheer Khan, Daniel J Park, PCR Protocols