Natural Killer Cell Protocols Cellular and Molecular Methods 2nd Edition by Aharon G Freud, Michael A Caligiur, Kerry S Campbell ISBN 1607613611 9781607613619

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ISBN 10: 1607613611 
ISBN 13: 9781607613619
Author: Aharon G Freud, Michael A Caligiur, Kerry S Campbell

As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of “Natural Killer Cell Protocols: Cellular and Molecular Methods” brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Natural Killer Cell Protocols Cellular and Molecular Methods 2nd Table of contents:

NK Cell Development and Maturation Analysis

• Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
  Aharon G. Freud, Michael A. Caligiuri (Pages 1-14)
• In Vitro Development of Human Killer–Immunoglobulin Receptor-Positive NK Cells
  Frank Cichocki, Jeffrey S. Miller (Pages 15-26)
• Subset Analysis of Human and Mouse Mature NK Cells
  Yoshihiro Hayakawa, Daniel M. Andrews, Mark J. Smyth (Pages 27-38)
• Assessing Licensing of NK Cells
  A. Helena Jonsson, Wayne M. Yokoyama (Pages 39-49)

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2. NK Cell Receptor Signaling and Activation

• Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo
  Rebecca H. Lian, Vinay Kumar (Pages 51-65)
• Use of Transfected Drosophila S2 Cells to Study NK Cell Activation
  Michael E. March, Catharina C. Gross, Eric O. Long (Pages 67-88)
• A Model System for Studying NK Cell Receptor Signaling
  Lukasz K. Chlewicki, Vinay Kumar (Pages 177-198)
• Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands
  Dikla Lankry, Roi Gazit, Ofer Mandelboim (Pages 249-273)
• Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation
  Alon Zilka, Michal Mendelson, Benyamin Rosental, Oren Hershkovitz, Angel Porgador (Pages 275-283)
• Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
  (No authors mentioned)

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3. NK Cell Interactions with Other Cells

• Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry
  Deborah N. Burshtyn, Chelsea Davidson (Pages 89-96)
• Studying NK Cell/Dendritic Cell Interactions
  Mathias Lucas, Cedric Vonarbourg, Peter Aichele, Andreas Diefenbach (Pages 97-126)
• Analysis of the NK Cell Immunological Synapse
  Keri B. Sanborn, Gregory D. Rak, Ashley N. Mentlik, Pinaki P. Banerjee, Jordan S. Orange (Pages 127-148)

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4. Intracellular Signaling and Measurement Methods

• Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry
  Alexander W. MacFarlane IV, James F. Oesterling, Kerry S. Campbell (Pages 149-157)
• Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells
  Takuya Miyagi, Seung-Hwan Lee, Christine A. Biron (Pages 159-175)

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5. Gene Editing and Expression in NK Cells

• Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction
  S. M. Shahjahan Miah, Kerry S. Campbell (Pages 199-208)
• Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
  Ram Savan, Tim Chan, Howard A. Young (Pages 209-221)
• Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus
  Amanda K. Purdy, Kerry S. Campbell (Pages 223-231)
• Introduction of shRNAs into Primary NK Cells with Lentivirus
  Sam K.P. Kung (Pages 233-247)

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Tags: Aharon G Freud, Michael A Caligiur, Kerry S Campbell, Natural Killer, Cell Protocols, Molecular Methods