Nanoparticles in Biology and Medicine Methods and Protocols 1st Edition by Claudia Gutiérrez Wing, Mikhail Soloviev ISBN 1493962477 9781493962471

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ISBN 10: 1493962477
ISBN 13: 9781493962471
Author: Claudia Gutiérrez Wing, Mikhail Soloviev

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ’70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery. In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.

Nanoparticles in Biology and Medicine Methods and Protocols 1st Edition Table of contents:

Part I: Noble Metal Nanoparticles: Synthesis and Applications

1. Procedures for the Synthesis and Capping of Metal Nanoparticles

Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán

2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications

Yeon-Su Park

3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis

Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan

4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays

Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev

5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues

Sadaki Yokota

6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics

Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista

7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins

Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev

Part II: Semiconductor Nanoparticles: Synthesis and Applications

8. Synthesis of Semiconductor Nanoparticles

Xianfeng Chen and Peter J. Dobson

9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature

Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba

10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods

Ana Sofia Miguel, Christopher Maycock, and Abel Oliva

11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing

Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides

12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis

Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, and Yury Rochev

13. The Use of Quantum Dots for Immunochemistry Applications

Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, and Carme Nogués

14. Quantum Dots for Labelling Live Cells

Yun Chen

15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots

Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer

16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

Julian Bailes and Mikhail Soloviev

Part III: Magnetic Nanoparticles: Synthesis and Applications

17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging

Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu

18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging

Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero

19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry

Maik Liebl and Dietmar Eberbeck

20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants

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