Nanoimaging Methods and Protocols 1st Edition by Alioscka Sousa ISBN 1493959611 9781493959617
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ISBN 10: 1493959611
ISBN 13: 9781493959617
Author: Alioscka Sousa
For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution. The book’s four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography; and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging. Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.
Nanoimaging Methods and Protocols 1st Edition Table of contents:
Chapter 1: Introduction to Nanoimaging
- 1.1 What is Nanoimaging?
- 1.2 History and Evolution of Nanoimaging Techniques
- 1.3 Key Principles Behind Nanoimaging
- 1.4 Applications in Science and Industry
- 1.5 Future Directions in Nanoimaging
Chapter 2: Principles of Imaging at the Nanoscale
- 2.1 Resolution and Contrast at the Nanoscale
- 2.2 Physical Principles of Nanoimaging
- 2.3 Challenges and Limitations
- 2.4 Advances in Nanoscale Imaging Technologies
Chapter 3: Scanning Probe Microscopy (SPM)
- 3.1 Atomic Force Microscopy (AFM)
- 3.2 Scanning Tunneling Microscopy (STM)
- 3.3 Techniques and Instrumentation
- 3.4 Imaging and Characterization Protocols
- 3.5 Applications in Nanomaterials
Chapter 4: Electron Microscopy Techniques
- 4.1 Transmission Electron Microscopy (TEM)
- 4.2 Scanning Electron Microscopy (SEM)
- 4.3 Scanning Transmission Electron Microscopy (STEM)
- 4.4 Cryo-Electron Microscopy (Cryo-EM)
- 4.5 Sample Preparation and Imaging Protocols
Chapter 5: X-ray Imaging and Diffraction
- 5.1 X-ray Crystallography for Nanoscale Studies
- 5.2 X-ray Microtomography
- 5.3 Synchrotron Radiation for Nanoimaging
- 5.4 Imaging Protocols and Sample Preparation
Chapter 6: Fluorescence and Confocal Microscopy
- 6.1 Principles of Fluorescence Imaging
- 6.2 Super-Resolution Microscopy Techniques
- 6.3 Fluorescence Resonance Energy Transfer (FRET)
- 6.4 Confocal and Multi-photon Microscopy
- 6.5 Advanced Fluorescence Imaging Protocols
Chapter 7: Raman Spectroscopy and Imaging
- 7.1 Principles of Raman Spectroscopy
- 7.2 Raman Imaging and Mapping Techniques
- 7.3 Surface-Enhanced Raman Spectroscopy (SERS)
- 7.4 Applications in Nanoimaging
Chapter 8: Computational Approaches for Nanoimaging
- 8.1 Image Processing and Analysis
- 8.2 Computational Modeling in Nanoimaging
- 8.3 Machine Learning in Nanoimaging
- 8.4 Data Visualization Techniques
Chapter 9: Sample Preparation Techniques for Nanoimaging
- 9.1 Sample Fixation and Staining
- 9.2 Thin Film Preparation
- 9.3 Cryo-immersion and Cryo-sectioning
- 9.4 Considerations for Biological vs. Material Samples
Chapter 10: Applications of Nanoimaging
- 10.1 Nanomaterials and Nanostructures
- 10.2 Biological and Medical Applications
- 10.3 Nanotechnology in Electronics and Energy
- 10.4 Environmental and Sustainability Research
Chapter 11: Emerging Trends and Future Directions
- 11.1 Next-Generation Nanoimaging Techniques
- 11.2 Hybrid Imaging Approaches
- 11.3 Integration with Other Analytical Techniques
- 11.4 Challenges and Ethical Considerations
- 11.5 The Future of Nanoimaging in Research
Appendix
- A1: List of Commonly Used Nanoimaging Instruments
- A2: Glossary of Terms
- A3: Troubleshooting Guide
- A4: Further Reading and Resources
Index
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