Mycobacteria Protocols Methods in Molecular Biology 465 2nd Edition by Tanya Parish ,Amanda Claire Brown ISBN 9781588298898 978-1588298898

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Product details: 

ISBN 10:9781588298898 

ISBN 13:978-1588298898

Author:Tanya Parish ,Amanda Claire Brown

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.

Table of contents: 

Isolation of Mycobacterium Species Genomic DNA
Isolation of Mycobacterial RNA
Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization
Analyzing Lipid Metabolism: Activation and β-Oxidation of Fatty Acids
Analysis of Lipid Biosynthesis and Location
Whole Genome Analysis Using Microarrays
Use of DNA Microarrays to Study Global Patterns of Gene Expression
Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria
Transport Assays and Permeability in Pathogenic Mycobacteria
Continuous Culture of Mycobacteria
Measuring Minimum Inhibitory Concentrations in Mycobacteria
Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts
Electroporation of Mycobacteria
Ins and Outs of Mycobacterial Plasmids
The Use of Temperature-Sensitive Plasmids in Mycobacteria
Heterologous Expression of Genes in Mycobacteria
Inducible Expression Systems for Mycobacteria
Assaying Promoter Activity Using LacZ and GFP as Reporters
Construction of Unmarked Deletion Mutants in Mycobacteria

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Tags: Tanya Parish, Amanda Claire Brown, Mycobacteria, Protocols