Mycobacteria Protocols 3rd Edition by Tanya Parish, David M Roberts ISBN 1493924494 9781493924493
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Product details:
ISBN 10: 1493924494
ISBN 13: 9781493924493
Author: Tanya Parish, David M Roberts
Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.
Mycobacteria Protocols 3rd Table of contents:
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Isolation of Mycobacterium Species Genomic DNA
Detailed protocols for extracting high-quality genomic DNA from various mycobacterial species. -
Isolation of Mycobacterial RNA
Methods for isolating RNA suitable for downstream applications like RT-PCR and transcriptomic analyses. -
Lipoglycans of Mycobacterium tuberculosis: Isolation, Purification, and Characterization
Techniques for isolating and analyzing lipoglycans, including lipoarabinomannan, which are critical components of the mycobacterial cell wall. -
Analyzing Lipid Metabolism: Activation and β-Oxidation of Fatty Acids
Protocols for studying the activation and β-oxidation processes of fatty acids in mycobacteria. -
Analysis of Lipid Biosynthesis and Location
Methods to investigate the biosynthesis pathways and localization of lipids within mycobacterial cells. -
Whole Genome Analysis Using Microarrays
Approaches for conducting comprehensive genome analyses using microarray technology. -
Use of DNA Microarrays to Study Global Patterns of Gene Expression
Techniques for utilizing DNA microarrays to examine global gene expression patterns in mycobacteria. -
Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria
Protocols for proteomic analysis using two-dimensional gel electrophoresis to study mycobacterial proteins. -
Transport Assays and Permeability in Pathogenic Mycobacteria
Methods to assess the transport mechanisms and permeability characteristics of pathogenic mycobacteria. -
Continuous Culture of Mycobacteria
Techniques for maintaining continuous cultures of mycobacteria for experimental purposes. -
Measuring Minimum Inhibitory Concentrations in Mycobacteria
Protocols for determining the minimum inhibitory concentrations of antimicrobial agents against mycobacteria. -
Rapid Screening of Inhibitors of Mycobacterium tuberculosis Growth Using Tetrazolium Salts
Methods for quickly screening potential inhibitors of M. tuberculosis growth using tetrazolium salts. -
Electroporation of Mycobacteria
Techniques for introducing DNA into mycobacterial cells via electroporation. -
Ins and Outs of Mycobacterial Plasmids
Discussion on the use and manipulation of plasmids in mycobacterial research. -
The Use of Temperature-Sensitive Plasmids in Mycobacteria
Protocols for employing temperature-sensitive plasmids to study gene function in mycobacteria. -
Heterologous Expression of Genes in Mycobacteria
Methods for expressing foreign genes in mycobacterial hosts. -
Inducible Expression Systems for Mycobacteria
Techniques for controlling gene expression in mycobacteria using inducible systems. -
Assaying Promoter Activity Using LacZ and GFP as Reporters
Protocols for measuring promoter activity using LacZ and GFP reporter genes. -
Construction of Unmarked Deletion Mutants in Mycobacteria
Methods for creating unmarked gene deletion mutants in mycobacteria. -
Construction of Targeted Mycobacterial Mutants by Homologous Recombination
Techniques for generating targeted mutants through homologous recombination. -
Phage Transposon Mutagenesis
Protocols for inducing mutations using phage transposons in mycobacteria. -
Gene Essentiality Testing in Mycobacterium smegmatis Using Specialized Transduction
Methods for assessing gene essentiality in M. smegmatis through specialized transduction. -
Gene Switching and Essentiality Testing
Techniques for switching genes and testing their essentiality in mycobacteria. -
Insertion Element IS6110-Based Restriction Fragment Length Polymorphism Genotyping of Mycobacterium tuberculosis
Protocols for genotyping M. tuberculosis using IS6110-based RFLP analysis. -
Typing Mycobacterium tuberculosis Using Variable Number Tandem Repeat Analysis
Methods for typing M. tuberculosis strains through VNTR analysis. -
Molecular Detection of Drug-Resistant Mycobacterium tuberculosis with a Scanning-Frame Oligonucleotide Microarray
Techniques for detecting drug-resistant M. tuberculosis strains using microarray technology. -
MycoDB: An Online Database for Comparative Genomics of the Mycobacteria and Related Organisms
Overview of MycoDB, a resource for comparative genomics of mycobacteria. -
Environmental Amoebae and Mycobacterial Pathogenesis
Discussion on the interaction between environmental amoebae and mycobacterial pathogenesis.
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