Mouse Cell Culture Methods and Protocols 1st Edition by Melany Jackson ISBN 1597450197 9781597450195

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ISBN 10: 1597450197
ISBN 13: 9781597450195
Author: Melany Jackson

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Mouse Cell Culture Methods and Protocols 1st Table of contents:

1. Introduction to Mouse Cell Culture

• Overview of Mouse Cell Types

• Applications in Research

• Basic Laboratory Setup and Safety

2. Preparation and Maintenance of Mouse Primary Cells

• Isolation of Mouse Fibroblasts

• Culture of Mouse Epithelial Cells

• Culturing Mouse Mesenchymal Stem Cells

3. Mouse Immune Cell Culture Protocols

• Isolation and Culture of Mouse Neutrophils

• Mouse B Cell Culture Methods

• Culturing Mouse T Cells and Macrophages

4. Mouse Embryonic Stem Cell Culture

• Derivation of Mouse Embryonic Stem Cells

• Maintenance and Differentiation Protocols

• Genetic Manipulation Techniques

5. Mouse Cancer Cell Lines and Models

• Culturing Mouse Tumor Cell Lines

• 3D Culture and Spheroid Models

• Co-culture Systems for Tumor Microenvironment

6. Media, Supplements, and Reagents

• Selecting Appropriate Culture Media

• Growth Factors and Cytokines

• Antibiotics and Antimycotics

7. Techniques for Assessing Cell Viability and Function

• Cell Counting and Viability Assays

• Flow Cytometry Protocols

• Functional Assays for Immune Cells

8. Troubleshooting Common Issues in Mouse Cell Culture

• Contamination Prevention and Management

• Optimizing Growth Conditions

• Dealing with Cellular Senescence

9. Advanced Culture Techniques

• 3D Culture and Organoid Systems

• Co-culture and Mixed Cell Populations

• High-Throughput Screening Adaptations

10. Ethical and Regulatory Considerations

• Animal Welfare in Cell Culture Research

• Biosafety Guidelines

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Tags: Melany Jackson, Mouse Cell, Protocols