Molecular Diagnostics of Infectious Diseases 2nd Edition by Harald Kessler 9783110278927 3110278928

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ISBN 10: 3110278928
ISBN 13: 9783110278927 
Author: Harald H. Kessler

The prevalence of infectious diseases is worldwide increasing. Therefore, detection methods for infectious pathogens change quickly. In the new edition of Kessler´s Molecular Diagnostics of Infectious Diseases laboratory professionalists get valueable information about the current diagnostic methods, tipps and tricks in terms of sample processing, quality control, and interpretation of the results. For clinicians the book is a valuable aid for decision-making in ordering appropriate tests as well as in assuring the necessary quality of the sample material.

Molecular Diagnostics of Infectious Diseases 2nd Table of contents:

1. Choice of adequate sample material
1.1. Viruses
1.2. Bacteria
1.3. Fungi
1.4. Protozoa

2. Stability of the specimen during preanalytics
2.1. Degradation of DNA
2.2. Degradation of RNA
2.3. Inhibitors of PCR
2.4. How can contamination during specimen collection and in the laboratory be avoided?
2.5. How can the sample identity be ensured?
2.6. Transport of diagnostic material
2.7. Stability of nucleic acids of selected pathogens during preanalytics
2.8. Take-home messages
2.9. Further reading

3. Quality assurance and quality control
3.1. Accreditation issues
3.2. Validation and verification work
3.3. Components of validation work
3.4. Components of verification work
3.5. Take-home messages
3.6. Further reading

4. Extraction of nucleic acids
4.1. Manual nucleic acid extraction protocols
4.2. Automated nucleic acid extraction platforms
4.3. Preparation of PCR mixes and addition of eluates
4.4. Currently frequently used commercially available platforms for nucleic acid extraction
4.5. Take-home messages
4.6. Further reading

5. Amplification and detection methods
5.1. Nucleic acid-based tests
5.2. Target amplification methods
5.3. Signal amplification methods
5.4. What are the key challenges for the future?
5.5. Take-home messages
5.6. Further reading

6. Interpreting and reporting molecular diagnostic tests
6.1. Qualitative and quantitative detection of viral infections
6.2. Detection of bacterial infections
6.3. Quantitative endpoint PCR
6.4. Real-time PCR
6.5. Reporting results
6.6. Interpretation
6.7. Important issues when clinically interpreting molecular diagnostic results
6.8. Take-home messages
6.9. Further reading

7. Human immunodeficiency virus
7.1. Major symptoms
7.2. Preanalytics
7.3. Analytics
7.4. Postanalytics
7.5. Take-home messages
7.6. Further reading

8. Hepatitis viruses
8.1. Major symptoms
8.2. Preanalytics
8.3. Analytics
8.4. Postanalytics – interpretation of results
8.5. Take-home messages
8.6. Further reading

9. Pathogens relevant in transplantation medicine
9.1. Clinical manifestations
9.2. Preanalytics
9.3. Analytics
9.4. Postanalytics – interpretation of results
9.5. Take-home messages
9.6. Further reading

10. Pathogens in lower respiratory tract infections
10.1. Clinical importance of different etiologic agents
10.2. Specimen collection
10.3. Diagnostic procedures
10.4. External quality control
10.5. The clinical usefulness and implementation of NAATs
10.6. Concluding remarks
10.7. Further reading

11. Molecular diagnosis of gastrointestinal pathogens
11.1. Clinical manifestations
11.2. Preanalytics
11.3. Analytics
11.4. Postanalytics
11.5. Further reading

12. Pathogens relevant in the central nervous system
12.1. Clinical manifestations
12.2. Preanalytics
12.3. Analytics
12.4. Postanalytics
12.5. Conclusion
12.6. Take-home messages
12.7. Acknowledgment
12.8. Further reading

13. Pathogens relevant in sexually transmitted infections
13.1. Symptoms and clinical manifestations
13.2. Preanalytics
13.3. Analytics
13.4. Postanalytics
13.5. Further reading

14. Index

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Tags: Harald Kessler, Molecular, Diagnostics