Microtubule Dynamics Methods and Protocols Methods in Molecular Biology Vol 777 1st Edition by Anne Straube ISBN 1617792519 9781617792519

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ISBN 10: 1617792519 
ISBN 13: 9781617792519
Author: Anne Straube

Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back.  In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Microtubule Dynamics Methods and Protocols Methods in Molecular Biology Vol 777 1st Table of contents:

How to Measure Microtubule Dynamics?
Anne Straube
Pages 1-14
Purification of Tubulin from Porcine Brain
Christopher Gell, Claire T. Friel, Barbara Borgonovo, David N. Drechsel, Anthony A. Hyman, Jonathon Howard
Pages 15-28
Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe
Douglas R. Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, Robert A. Cross
Pages 29-55
Generation of Differentially Polyglutamylated Microtubules
Benjamin Lacroix, Carsten Janke
Pages 57-69
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Laurence Lafanechère, Didier Job
Pages 71-86
αβ-Tubulin and Microtubule-Binding Assays
Jaime N. Campbell, Kevin C. Slep
Pages 87-97
Analysis of Microtubule Assembly Kinetics Using Turbidimetry
Felicia Gaskin
Pages 99-105
Isolation of Centrosomes from Cultured Cells
Simone Reber
Pages 107-116
Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds
Miho Katsuki, Etsuko Muto, Robert A. Cross
Pages 117-126
Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
Ivo A. Telley, Peter Bieling, Thomas Surrey
Pages 127-145
Force Generation by Dynamic Microtubules In Vitro
Svenja-Marei Kalisch, Liedewij Laan, Marileen Dogterom
Pages 147-165
Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
Susanne Bechstedt, Michal Wieczorek, Michael Noujaim, Gary J. Brouhard
Pages 167-176
Analysing the ATP Turnover Cycle of Microtubule Motors
Claire T. Friel, Clive R. Bagshaw, Jonathon Howard
Pages 177-192
Cryo-electron Tomography of Microtubules Assembled In Vitro from Purified Components
Frédéric M. Coquelle, Sophie Blestel, Claire Heichette, Isabelle Arnal, Charles Kervrann, Denis Chrétien
Pages 193-208
Kinetochore Microtubules, Analyzed by Correlated Light and Immunoelectron Microscopy
Andreas Merdes, Jan De Mey
Pages 209-221
Photoactivatable-GFP-α-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells
Catarina P. Samora, Andrew D. McAinsh
Pages 223-233
Quantification of Asymmetric Microtubule Nucleation at Subcellular Structures
Xiaodong Zhu, Irina Kaverina
Pages 235-244
Investigating Microtubule Dynamic Instability Using Microtubule-Targeting Agents
Stéphane Honore, Diane Braguer
Pages 245-260
Laser Ablation of the Microtubule Cytoskeleton: Setting Up and Working with an Ablation System
Nicola Maghelli, Iva M. Tolić-Nørrelykke
Pages 261-271

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Tags: Anne Straube, Microtubule Dynamics, Protocols Methods