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Microinjection Methods and Applications 1st Edition by Jill C Sible, Brian N Wroble, David J Carroll ISBN 1588298841 9781588298843

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Microinjection Methods and Applications 1st Edition Jill C. Sible Digital Instant Download

Author(s): Jill C. Sible, Brian N. Wroble (auth.), David J. Carroll (eds.)
ISBN(s): 9781588298843, 1588298841
Edition: 1
File Details: PDF, 2.99 MB
Year: 2009
Language: english
SKU: EB-1315620 Category: Tags: , ,

Microinjection Methods and Applications 1st Edition by Jill C Sible, Brian N Wroble, David J Carroll – Ebook PDF Instant Download/Delivery: 1588298841, 9781588298843
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ISBN 10: 1588298841 
ISBN 13: 9781588298843
Author: Jill C Sible, Brian N Wroble, David J Carroll

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.

Microinjection Methods and Applications 1st Table of contents:

Chapter 1: Introduction to Microinjection

  • Definition and Overview of Microinjection

  • Historical Development of Microinjection Techniques

  • Key Advances in Microinjection Technology

  • Importance in Research and Biotechnology

Chapter 2: Basic Principles of Microinjection

  • Physics and Mechanics of Microinjection

  • Micromanipulation Tools and Equipment

  • Injection Techniques: Manual vs. Automated

  • Choosing the Right Micropipette

Chapter 3: Equipment and Setup for Microinjection

  • Microscopes and Imaging Systems

  • Injection Pumps and Pressure Regulators

  • Micromanipulators and Holders

  • Sterilization and Maintenance of Equipment

Chapter 4: Microinjection Techniques in Animal Cells

  • Introduction to Animal Cell Microinjection

  • Techniques for Injecting DNA, RNA, Proteins, and Other Molecules

  • Challenges and Solutions in Animal Cell Microinjection

  • Applications in Transfection, Gene Editing, and Protein Studies

Chapter 5: Microinjection in Embryology and Developmental Biology

  • Microinjection in Embryos: Early Development Stages

  • Gene Editing and Manipulation in Embryos

  • Applications in Transgenesis and Knockout Models

  • Ethical Considerations in Embryonic Research

Chapter 6: Microinjection Techniques in Plant Cells

  • Challenges of Plant Cell Microinjection

  • Techniques for Introducing DNA, RNA, and Proteins into Plant Cells

  • Applications in Plant Biotechnology and Genetic Engineering

Chapter 7: Applications of Microinjection in Genetic Engineering

  • Gene Therapy and Microinjection of Therapeutic DNA

  • Stem Cell Research and Microinjection Applications

  • CRISPR-Cas9 and Other Gene Editing Technologies

  • Future Directions in Genetic Engineering via Microinjection

Chapter 8: Troubleshooting and Optimization of Microinjection

  • Common Issues in Microinjection Procedures

  • Tips for Improving Success Rates

  • Ensuring High Efficiency in Injecting Targeted Cells

  • Troubleshooting Equipment and Technique Problems

Chapter 9: Advanced Microinjection Methods

  • Automated and High-Throughput Microinjection Systems

  • Single-Cell Manipulation and Microinjection

  • Multi-Particle Injection Techniques

  • Innovations in Nanotechnology for Microinjection

Chapter 10: Ethical and Regulatory Considerations in Microinjection

  • Ethical Implications in Animal and Human Research

  • Regulatory Guidelines and Compliance

  • Handling and Disposal of Biological Samples

  • Best Practices for Ethical Microinjection Research

Chapter 11: Future Directions in Microinjection Technology

  • Emerging Technologies and Innovations in Microinjection

  • Trends in Single-Cell and Multi-Omics Analysis

  • Integration of Microinjection with Other Genetic Engineering Techniques

  • The Future of Microinjection in Medicine and Biotechnology

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Tags: Jill C Sible, Brian N Wroble, David J Carroll, Microinjection Methods