Microbial Toxins Methods and Protocols 1st Edition by Gabriella Pocsfalvi, Gitta Schlosser, Otto Holst ISBN 1617791024 9781617791024

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ISBN 10: 1617791024 
ISBN 13: 9781617791024
Author: Gabriella Pocsfalvi, Gitta Schlosser, Otto Holst

This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Microbial Toxins Methods and Protocols 1st Table of contents:

Detection of Cholera Toxin by an Immunochromatographic Test Strip
Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Biswajit Maiti, Kayo Okumura, Fumiki Morimatsu et al.
Pages 1-7
Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
Jessica Daniel, Lisa Fetter, Susan Jett, Teisha J. Rowland, Andrew J. Bonham
Pages 9-23
A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis
Patrick Cherubin, Beatriz Quiñones, Salem Elkahoui, Wallace Yokoyama, Ken Teter
Pages 25-36
Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin
Basavraj Nagoba, Mahesh Dharne, Kushal N. Gohil
Pages 37-47
Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food
Alexandra Morlay, Agnès Roux, Vincent Templier, Félix Piat, Yoann Roupioz
Pages 49-59
Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection
Christin Fischer, Markus Fischer
Pages 61-68
Detection of Yersinia pestis in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry
Jérôme Chenau, François Fenaille, Stéphanie Simon, Sofia Filali, Hervé Volland, Christophe Junot et al.
Pages 69-83
A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin-LR
Shu-Chi Chang, Bo-Li Lu, Jiang-Jen Lin, Yen-Hsien Li, Maw-Rong Lee
Pages 85-94
An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaneously
Xiaofei Hu, Gaiping Zhang
Pages 95-105
Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System
Janusz Boratyński, Bożena Szermer-Olearnik
Pages 107-112
Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins
Paulina Żarnowiec, Grzegorz Czerwonka, Wiesław Kaca
Pages 113-124
Laser Interferometry Method as a Novel Tool in Endotoxins Research
Michał Arabski, Sławomir Wąsik
Pages 125-132
Endotoxin Entrapment on Glass via C-18 Self-Assembled Monolayers and Rapid Detection Using Drug-Nanoparticle Bioconjugate Probes
Prasanta Kalita, Anshuman Dasgupta, Shalini Gupta
Pages 133-142
A Bioassay for the Determination of Lipopolysaccharides and Lipoproteins
Marcus Peters, Petra Bonowitz, Albrecht Bufe
Pages 143-150
Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes and Subclasses
Béla Kocsis, Lilla Makszin, Anikó Kilár, Zoltán Péterfi, Ferenc Kilár
Pages 151-165
Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides
Alexey Novikov, Aude Breton, Martine Caroff
Pages 167-186
Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures
Béla Kocsis, Anikó Kilár, Szandra Péter, Ágnes Dörnyei, Viktor Sándor, Ferenc Kilár
Pages 187-198

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