Microarray Technology Through Applications 1st Edition by Francesco Falciani – Ebook PDF Instant Download/Delivery: 0415378532, 9780415378536
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ISBN 10: 0415378532
ISBN 13: 9780415378536
Author: Francesco Falciani
Microarray Technology Through Applications 1st Table of contents:
1 Introduction to microarray technology
1.1 Introduction to the technology and its applications
1.2 The design of a microarray
1.3 Glass slide DNA microarrays
1.4 Affymetrix microarrays
1.5 Microarray platforms for protein studies and other applications
1.6 Data/image acquisition
Acknowledgments
References
2 Immunoprecipitation with microarrays to determine the genomewide binding profile of a DNA-associated protein
2.1 Introduction
2.2 Background
2.3 Experimental design
2.4 Data acquisition
2.5 Theory of data analysis
2.6 Data analysis
2.7 Summary of the results, conclusions, and related applications
References
3 Array-based comparative genomic hybridization as a tool for solving practical biological and medical questions
3.1 Introduction
3.2 Scientific background
3.3 Design of the experiments
3.4 Data acquisition
3.5 Theory of data analysis
3.6 Data analysis
3.7 Summary of the results
3.8 Conclusions and suggestions for the general implementation of the case study
Acknowledgments
References
4 Use of single nucleotide polymorphism arrays: Design, tools, and applications
4.1 Introduction
4.2 Scientific background: Essential steps to be considered in the design of the experiment
4.3 Use of microarray technology: The Illumina platform as an example
4.4 Tools for data acquisition and data analysis
4.5 Summary and conclusions
Acknowledgments
References
5 In vitro analysis of gene expression
5.1 Introduction
5.2 Scientific background
5.3 Design of the experiment
5.4 Data acquisition
5.5 Theory of data analysis
5.6 Data analysis
5.7 Summary of results, conclusions, and suggestions for general implementation of the case study
References
6 The analysis of cellular transcriptional response at the genome level: Two case studies with relevance to bacterial pathogenesis
6.1 Introduction
6.2 Scientific background
6.3 Design of the experiment
6.4 A description of data analysis procedures
6.5 Data analysis tutorials
6.6 Results and discussion
6.7 Conclusions
Acknowledgments
References
7 Functional annotation of microarray experiments
7.1 Introduction
7.2 Scientific background
7.3 Design of the experiment
7.4 Theory of data analysis
7.5 Data analysis
7.6 Summary of the results, conclusions, and suggestions for the general implementation of the case study
Acknowledgments
References
8 Microarray technology in agricultural research
8.1 Introduction
8.2 Microarray resources in agricultural research
8.3 Home-made plant microarrays
8.4 Microarrays and genetically modified organisms
8.5 A data analysis example: Time course of gene expression response to stress in transgenic plants
8.6 Concluding remarks
References
9 Protein microarrays
9.1 Introduction
9.2 The uses and application of protein microarrays
9.3 The practicalities of protein microarrays
9.4 Overall concluding remarks
References
Protocol 1: Printing oligonucleotide microarrays
Protocol 2: Extraction of E. coli RNA
Protocol 3: Probe labeling
Protocol 4: Hybridization and washing
Protocol 5: ChIP procedure
Protocol 6: Array comparative genomic hybridization (CGH)
Protocol 7: Run-off microarray analysis of gene expression (ROMA)
Protocol 8: Selection and genotyping of single nucleotide polymorphisms
Appendix 1: Notes on printing glass DNA microarray slides
Appendix 2: Comparative genomics. The nature of CGH analysis and data interpretation
Appendix 3: Useful web links to microarray resources
Appendix 4: The genes selected for clustering (Chapter 6, Figure 6.4)
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