Mast Cells Methods and Protocols 2nd Edition by Michael R Hughes, Kelly M McNagny ISBN 1493915673 9781493915675

Mast Cells Methods and Protocols 2nd Edition by Michael R Hughes, Kelly M McNagny – Ebook PDF Instant Download/Delivery: 1493915673, 9781493915675
Full download Mast Cells Methods and Protocols 2nd Edition after payment

Product details:

ISBN 10: 1493915673 
ISBN 13: 9781493915675
Author: Michael R Hughes, Kelly M McNagny

Mast Cells: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike.  With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues, Danio rerio (zebrafish) model in the study of mast cell development and function, the functions of mast cells in human health and disease and methods for the isolation, methods for the investigation of the molecular mechanisms of mast cell activation and their effector functions, assays for the detection and analysis of mast cell secretory and cell surface phenotype and, mast cell activation state, and experimental mouse models of disease that have been deemed useful for the assessment of mast cell functions in the regulation of innate and adaptive immune response in cancer, tissue fibrosis, auto-inflammation and allergicdisease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Mast Cells Methods and Protocols 2nd Table of contents:

Part I: Overview of Mast Cell Biology

• 1. Front Matter

• 2. Overview of Mast Cell Biology

  • Paul Ehrlich’s “Mastzellen” — From Aniline Dyes to DNA Chip Arrays

  • The Human Mast Cell

  • Mast Cells in Allergy and Autoimmunity

Part II: Identification of Mast Cells in Culture and Tissue

• Front Matter

• Analysis of MCT and MCTC Mast Cells in Tissue

• Mast Cell Ultrastructure and Tissue Staining

• Expression of Cell Surface Antigens on Mast Cells

• Identification of Mast Cells in the Cellular Response to Myocardial Infarction

Part III: Development of Mast Cells In Vitro

• Front Matter

• Growth of Human Mast Cells From Bone Marrow and Peripheral Blood‑Derived CD34+ Progenitor Cells

• Culture of Human Mast Cells From Hemopoietic Progenitors

• Isolation, Culture, and Characterization of Intestinal Mast Cells

Part IV: Mast Cell Signaling and Gene Expression

• Front Matter

• Activation of Nuclear Factor‑κB

• Analysis of Mitogen‑Activated Protein Kinase Activation

• Microarray and Gene‑Clustering Analysis

• Techniques to Study FcεRI Signaling

Part V: Mast Cell Expression and Inflammatory Mediators

• Front Matter

• (Detailed protocols likely covering histamine, cytokine release, protease assays – not explicitly listed but inferred based on series format)

Part VI: Mast Cell Interactions with Other Cell Types

• Endothelial Cell Activation by Mast Cell Mediators

• Co-Culture Techniques with Fibroblasts

• Detection of Class Switching and IgE Synthesis in Human B Cells

People also search for Mast Cells Methods and Protocols 2nd:

basophils and mast cells methods and protocols
    
types of mast cells
    
how to control mast cells
    
what are examples of mast cells
    
mast cells purpose

Tags: Michael R Hughes, Kelly M McNagny, Mast Cells