Mass Spectrometry of Glycoproteins Methods and Protocols 1st Edition by Arnaud Delobel ISBN 1071612409 9781071612408

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ISBN 10: 1071612409 
ISBN 13: 9781071612408
Author: Arnaud Delobel

This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide–, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Mass Spectrometry of Glycoproteins Methods and Protocols 1st Table of contents:

Glycosylation of Therapeutic Proteins: A Critical Quality Attribute
Arnaud Delobel
Pages 1-21
Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry
Dan Bach Kristensen, Trine Meiborg Sloth, Martin Ørgaard, Pernille Foged Jensen
Pages 23-45
Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Estela Giménez, Montserrat Mancera-Arteu, Fernando Benavente, Victoria Sanz-Nebot
Pages 47-56
Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS
Yosra Helali, Shilpee Sharma, Marie Vandeput, Dansala Welba, Pierre Van Antwerpen, Arnaud Marchant et al.
Pages 57-71
Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry
Elsa Wagner-Rousset, Olivier Colas, Stéphane Chenu, Yannis-Nicolas François, Davy Guillarme, Sarah Cianferani et al.
Pages 73-83
Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS
Valentina D’Atri, Davy Guillarme
Pages 85-95
Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis–Mass Spectrometry Coupling (CE-MS)
Josiane Saadé, Michael Biacchi, Jérémie Giorgetti, Antony Lechner, Alain Beck, Emmanuelle Leize-Wagner et al.
Pages 97-106
Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography
Abel Bermudez, Sharon J. Pitteri
Pages 107-120
In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC SPE and LC-MS/MS
Myung Jin Oh, Youngsuk Seo, Unyong Kim, Hyun Joo An
Pages 121-131
Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE
Kevin Canis, Estelle Garénaux, Jean-François Boe
Pages 133-154
Mapping O-glycosylation Sites Using OpeRATOR and LC-MS
Maria Nordgren, Andreas Nägeli, Helén Nyhlén, Jonathan Sjögren
Pages 155-167
Site-Specific O-Glycosylation Analysis by Liquid Chromatography–Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional Dissociation
Noritaka Hashii, Junya Suzuki
Pages 169-178
Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS After Derivatization with Fluorescent Anthranilamide
Claire I. Butré, Eric Largy, Arnaud Delobel
Pages 179-188
Evaluating N-Glycosylation of a Therapeutic Monoclonal Antibody Using UHPLC-FLR-MS with RapiFluor-MS Labeling
Rosie Upton, James Duffy, Sam Clawson, David Firth
Pages 189-203
Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection
Ximo Zhang, Vithiya Vimalraj, Mitul Patel
Pages 205-219
Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals
Sonja Schneider, Edgar Naegele, Sonja Krieger
Pages 221-235
Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS After Derivatization with Fluorescent Anthranilamide
Claire I. Butré, Eric Largy, Fabrice Cantais, Arnaud Delobel
Pages 237-247
Linkage Analysis of Oligosaccharides and Polysaccharides: A Tutorial
Ian Black, Christian Heiss, Russell W. Carlson, Parastoo Azadi
Pages 249-271
Use of Exoglycosidases for the Structural Characterization of Glycans
Elizabeth McLeod, Paula Magnelli, Xiaofeng Shi
Pages 273-280

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Tags: Arnaud Delobel, Mass Spectrometry, Glycoproteins Methods