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Liposomes Methods and Protocols Volume 2 Biological Membrane Models 1st Edition by Alfred Fahr, Xiangli Liu, Volkmar Weissig ISBN 1607614472 9781607614470

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Liposomes Methods and Protocols Volume 2 Biological Membrane Models 1st Edition Alfred Fahr Digital Instant Download

Author(s): Alfred Fahr, Xiangli Liu (auth.), Volkmar Weissig (eds.)
ISBN(s): 9781607614470, 1607614472
Edition: 1
File Details: PDF, 24.03 MB
Year: 2010
Language: english
SKU: EB-2014074 Category: Tags: , ,

Liposomes Methods and Protocols Volume 2 Biological Membrane Models 1st Edition by Alfred Fahr, Xiangli Liu, Volkmar Weissig – Ebook PDF Instant Download/Delivery: 1607614472, 9781607614470
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ISBN 10: 1607614472 
ISBN 13: 9781607614470
Author: Alfred Fahr, Xiangli Liu, Volkmar Weissig

Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that “the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.

Liposomes Methods and Protocols Volume 2 Biological Membrane Models 1st Table of contents:

1. Introduction to Biological Membrane Models

  • Overview of Liposome Models

  • Importance of Biological Membrane Mimics

2. Preparation of Liposomes for Membrane Studies

  • Methods for Liposome Formation

  • Lipid Composition and Selection

  • Size Control and Homogeneity

3. Characterization Techniques for Liposome Models

  • Dynamic Light Scattering (DLS)

  • Electron Microscopy

  • Fluorescence Spectroscopy

  • Nuclear Magnetic Resonance (NMR)

4. Liposome-Based Membrane Models

  • Supported Lipid Bilayers

  • Tethered Lipid Bilayers

  • Black Lipid Membranes

  • Giant Unilamellar Vesicles (GUVs)

5. Protein Incorporation into Liposome Models

  • Reconstitution of Membrane Proteins

  • Detergent Removal Techniques

  • Functional Assays of Membrane Proteins

6. Functional Studies Using Liposome Models

  • Ion Transport and Channel Activity

  • Fusion and Fission Mechanisms

  • Membrane Permeability Assays

7. Advanced Imaging and Spectroscopy Applications

  • Atomic Force Microscopy (AFM)

  • Confocal Microscopy of Liposomes

  • Fluorescence Resonance Energy Transfer (FRET)

8. Applications in Drug Delivery and Pharmacology

  • Liposome Stability and Drug Encapsulation

  • Targeting and Release Mechanisms

  • Liposomes in Therapeutics

9. Troubleshooting and Optimization

  • Common Challenges in Liposome Preparation

  • Tips for Reproducibility

  • Optimizing Experimental Conditions

10. Appendices

  • Recipes for Lipid Solutions

  • Protocol Variations

  • List of Reagents and Suppliers

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Tags: Alfred Fahr, Xiangli Liu, Volkmar Weissig, Liposomes Methods