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Lentivirus Gene Engineering Protocols 1st Edition by Maurizio Federico ISBN 1588290913 9781588290915

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Lentivirus Gene Engineering Protocols 1st Edition Maurizio Federico Digital Instant Download

Author(s): Maurizio Federico
ISBN(s): 9781588290915, 1588290913
Edition: 1
File Details: PDF, 5.93 MB
Year: 2003
Language: english
SKU: EB-921360 Category: Tag:

Lentivirus Gene Engineering Protocols 1st Edition by Maurizio Federico – Ebook PDF Instant Download/Delivery: 1588290913, 9781588290915
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ISBN 10: 1588290913 
ISBN 13: 9781588290915
Author: Maurizio Federico

Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.

Lentivirus Gene Engineering Protocols 1st Table of contents:

  1. Introduction to Lentiviral Vectors
    Overview of lentivirus biology and vector design principles.

  2. Production of Lentiviral Vectors
    Protocols for generating high-titer lentiviral stocks.

  3. Titration and Quality Control of Lentiviral Preparations
    Methods to quantify and ensure safety of viral vectors.

  4. Pseudotyping Lentiviral Vectors
    Techniques to alter viral tropism for targeting specific cell types.

  5. Transduction of Mammalian Cells
    Protocols for efficient gene delivery into various cell lines.

  6. Lentiviral Vector-Mediated RNA Interference
    Application of lentiviral vectors for gene knockdown using shRNA.

  7. Regulatable Lentiviral Systems
    Designing inducible expression systems for controlled gene expression.

  8. Lentiviral Vector Biosafety Considerations
    Guidelines for safe handling and use of lentiviral vectors.

  9. In Vivo Applications of Lentiviral Vectors
    Protocols for gene delivery in animal models.

  10. Analysis of Transgene Expression
    Methods to assess gene expression at RNA and protein levels.

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Tags: Maurizio Federico, Lentivirus Gene, Engineering Protocols