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JAK STAT Signalling Methods and Protocols 1st Edition by Jeremy A Ross, Georgialina Rodriguez, Robert A Kirken, Sandra E Nicholson, Nicos A Nicola ISBN 9781627032421 1627032428

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JAK STAT Signalling Methods and Protocols 1st Edition Jeremy A. Ross Digital Instant Download

Author(s): Jeremy A. Ross, Georgialina Rodriguez, Robert A. Kirken (auth.), Sandra E. Nicholson, Nicos A. Nicola (eds.)
ISBN(s): 9781627032421, 1627032428
Edition: 1
File Details: PDF, 4.05 MB
Year: 2013
Language: english
SKU: EB-4290162 Category: Tags: , , , ,

JAK STAT Signalling Methods and Protocols 1st Edition by Jeremy A Ross, Georgialina Rodriguez, Robert A Kirken, Sandra E Nicholson, Nicos A Nicola – Ebook PDF Instant Download/Delivery: 9781627032421 ,1627032428
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Product details:

ISBN 10: 1627032428
ISBN 13: 9781627032421
Author: Jeremy A Ross, Georgialina Rodriguez, Robert A Kirken, Sandra E Nicholson, Nicos A Nicola

The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. __JAK-STAT Signalling: Methods and Protocols__ provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful __Methods in Molecular BiologyTM__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, __JAK-STAT Signalling: Methods and Protocols__ will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.

JAK STAT Signalling Methods and Protocols 1st Edition Table of contents:

  1. Introduction to JAK–STAT Signaling

  2. Overview of JAK and STAT Family Members

  3. Cell Culture Systems for JAK–STAT Studies

  4. Stimulation Protocols: Cytokines and Growth Factors

  5. Western Blotting for JAK and STAT Proteins

  6. Immunoprecipitation and Kinase Assays

  7. Phospho-STAT Detection: Flow Cytometry and ELISA

  8. Immunofluorescence and Confocal Microscopy Applications

  9. Reporter Gene and Luciferase Assays

  10. Chromatin Immunoprecipitation (ChIP) for STAT Binding

  11. Quantitative PCR and Gene Expression Analysis

  12. RNA Interference and Gene Knockdown Strategies

  13. CRISPR/Cas9 Approaches for JAK–STAT Signaling

  14. In Vivo Mouse Models for Pathway Analysis

  15. STAT Activity in Disease Models (e.g. Cancer, Inflammation)

  16. High-Throughput Screening and Inhibitor Development

  17. Bioinformatic and Systems Biology Approaches

  18. Data Analysis and Standardization in JAK–STAT Research

  19. Troubleshooting Protocols

  20. Appendices

  • Reagent Preparation and Buffer Recipes

  • Antibody and Primer Listings

  • Safety and Biosafety Considerations

  1. References

  2. Index

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Tags: Jeremy A Ross, Georgialina Rodriguez, Robert A Kirken, Sandra E Nicholson, Nicos A Nicola, JAK STAT, Signalling