Influenza Virus Methods and Protocols 1st Edition by Yoshihiro Kawaoka, Gabriele Neumann – Ebook PDF Instant Download/Delivery: 1617796212, 9781617796210
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Product details:
ISBN 10: 1617796212
ISBN 13: 9781617796210
Author: Yoshihiro Kawaoka, Gabriele Neumann
This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Influenza Virus Methods and Protocols 1st Table of contents:
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Influenza Viruses – An Introduction
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Influenza Virus Isolation
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Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection
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Diagnosis of Influenza Virus
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Solid-phase Assays of Receptor-binding Specificity
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The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors
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The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors
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Animal Models
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Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture
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Genetic Engineering of Live Attenuated Influenza Viruses
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Influenza A Virus Molecular Virology Techniques
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Reverse Genetics of Influenza Viruses
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Genetic Analysis
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