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Handbook of size exclusion chromatography and related techniques 2nd Edition by Chi San Wu ISBN 0824747100 9780824747107

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Handbook of size exclusion chromatography and related techniques 2nd ed., rev. and expanded Edition Chi-San Wu Digital Instant Download

Author(s): Chi-San Wu
ISBN(s): 9780824747107, 0824747100
Edition: 2nd ed., rev. and expanded
File Details: PDF, 11.48 MB
Year: 2004
Language: english
SKU: EB-1203962 Category: Tag:

Handbook of size exclusion chromatography and related techniques 2nd Edition by Chi San Wu – Ebook PDF Instant Download/Delivery: 0824747100, 9780824747107
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ISBN 10: 0824747100 
ISBN 13: 9780824747107
Author: Chi San Wu

Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Handbook of size exclusion chromatography and related techniques 2nd Table of contents:

1 Introduction to Size Exclusion Chromatography
1 The SEC Experiment and Related Thermodynamics
2 Experimental Conditions for SEC
2.1 System Overview
2.2 Universal (Concentration) Detectors
2.3 Mobile Phase and Temperature
2.4 Stationary Phases
2.5 Sample Size and Mobile Phase Flow Rate
3 Calibration Methodology and Data Analysis in SEC
3.1 Direct Standard Calibration
3.2 Band Broadening Measurement and Correction
3.3 Polydisperse or Broad Standard Calibration
3.4 Universal Calibration
3.5 Molecular Weight Sensitive Detectors
3.5.1 Low Angle Laser Light Scattering Detection
3.5.2 Multi-Angle Laser Light Scattering Detection
3.5.3 Right-Angle Laser Light Scattering Detection and Triple Detection
3.5.4 Viscometric Detection
4 General References
5 Acknowledgements
6 References

2 Semirigid Polymer Gels for Size Exclusion Chromatography
1 Introduction
2 Column Packing and Performance
3 Organic SEC
3.1 Manufacture
3.2 Particle Size
3.3 Porosity
3.4 Mechanical and Chemical Stability
3.5 Column Selection/Applications
4 Aqueous SEC
4.1 Introduction
4.2 Porosity
4.3 Surface Chemistry
4.4 Eluent Selection
5 Conclusion
6 References

3 Modified Silica-Based Packing Materials for Size Exclusion Chromatography
1 Introduction
2 Properties of Silica
2.1 Structure, Synthesis, and Purity
2.2 Chromatographic Characteristics
2.3 Particle Morphology
2.4 Column Dimensions
2.5 Porosity
2.6 Surface Area
2.7 Silanol Groups
2.8 Deactivation
2.9 Chemical Modification
3 Calibration
4 Secondary Retention
5 Practical Considerations
5.1 Extracolumn Effects
5.2 Sample
5.3 Mobile Phase
5.4 Temperature
6 References

4 Molecular Weight Sensitive Detectors for Size Exclusion Chromatography
1 Introduction
2 Principles
2.1 Viscometry
2.2 Light Scattering
3 Methodology
3.1 Viscometry
3.1.1 Universal Calibration
3.1.2 SEC-Viscometry Without a Concentration Detector
3.1.3 Intrinsic Viscosity Distribution
3.1.4 Radius of Gyration Measurement
3.2 Light Scattering
3.2.1 Determination of the Specific Refractive Index Increment and Solvent Refractive Index
3.2.2 Instrument Calibration
3.2.3 Measurement of Molecular Weight Distribution
3.2.4 Measurement of Sample Mw
3.2.5 SEC-Light Scattering with Universal Calibration
3.2.6 Right-Angle Laser Light Scattering
3.3 Concentration Measurement
3.4 Interdetector Delay Volume
3.5 Band Broadening
4 Applications
4.1 Viscometry
4.1.1 Molecular Weight Distribution
4.1.2 Copolymer Molecular Weight Distribution
4.1.3 Branching
4.1.4 Mark–Houwink Coefficients
4.1.5 Biopolymer Characterization
4.2 Light Scattering
4.2.1 Molecular Weight Distribution
4.2.2 Copolymer Molecular Weight Distribution
4.2.3 Branching
4.2.4 Biopolymers
5 Special Applications
6 Summary
7 Acknowledgements
8 Appendix: Instrument Companies
8.1 Light-Scattering Detectors for SEC
8.2 Viscometers for SEC
9 References

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