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Genomic Imprinting Methods and Protocols 1st Edition by Nora Engel ISBN 1493959662 978-1493959662

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Genomic Imprinting Methods and Protocols 1st Edition Yong Cheng Digital Instant Download

Author(s): Yong Cheng, Dasari Amarnath, Keith E. Latham (auth.), Nora Engel (eds.)
ISBN(s): 9781627030113, 1627030115
Edition: 1
File Details: PDF, 4.34 MB
Year: 2012
Language: english
SKU: EB-4501876 Category: Tags: , , ,

Genomic Imprinting Methods and Protocols 1st Edition by Nora Engel – Ebook PDF Instant Download/Delivery: 1493959662, 978-1493959662

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Product details:

ISBN 10: 1493959662

ISBN 13: 978-1493959662 

Author:  Nora Engel 

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Prools, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Genomic Imprinting: Methods and Prools will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.

Table of contents:

  1. Uniparental Embryos in the Study of Genomic Imprinting

  2. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species

  3. Generation of Trophoblast Stem Cells

  4. Immunomagnetic Purification of Murine Primordial Germ Cells

  5. Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA

  6. Identification of Imprinted Loci by Transcriptome Sequencing

  7. Data Mining as a Discovery Tool for Imprinted Genes

  8. Engineering of Large Deletions and Duplications In Vivo

  9. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

  10. Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains

  11. Quantitative Chromosome Conformation Capture

  12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing

  13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies

  14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

  15. Co-Immunoprecipitation of Long Non-Coding RNAs

  16. Specialized Technologies for Epigenetics in Plants

  17. Computational Studies of Imprinted Genes

  18. Insights on Imprinting from Beyond Mice and Men

  19. Non-Mammalian Parent-Of-Origin Effects

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Tags: Nora Engel, Genomic, Imprinting, Methods, Protocols