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Gene Biotechnology 3rd Edition by William Wu, Helen H Zhang, Michael J Welsh, Peter B Kaufman ISBN 1439848300 9781439848302

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Gene Biotechnology Third Edition William Wu Digital Instant Download

Author(s): William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman
ISBN(s): 1439848300
Edition: 3
File Details: PDF, 10.86 MB
Year: 2011
Language: english
SKU: EB-2256444 Category: Tags: , , ,

Gene Biotechnology 3rd Edition by William Wu, Helen H Zhang, Michael J Welsh, Peter B Kaufman – Ebook PDF Instant Download/Delivery: 1439848300, 9781439848302
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Product details:

ISBN 10: 1439848300 
ISBN 13: 9781439848302
Author: William Wu, Helen H Zhang, Michael J Welsh, Peter B Kaufman

Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today’s biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ

Gene Biotechnology 3rd Table of contents:

1 Strategies for Novel Research Projects and/or Research Grant Funding
2 Rapid Isolation of Specific cDNAs orGenes by PCR
3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries
4 Subcloning of Genes or DNA Fragments
5 Nonisotopic and Isotopic DNA or RNA Sequencing
6 Bioinformation Superhighway and Computer Databases of Nucleic Acids
7 Characterization of DNA or Genes by Southern Blot Hybridization
8 Gene Overexpression by Sense RNA inMammalian Systems
9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
10 Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR
11 Analysis of Gene Expression at Proteomic Level via Western Blotting
12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization
13 Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization
14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs
15 Isolation and Characterization of Genes from Genomic DNA Libraries
16 Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression
17 Strategies for Gene Double Knockout
18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
19 Quantitative Analysis of Functional Genome by Real-Time RT-PCR
20 High-Throughput Analysis of Gene Expression by CuttingEdge Technology—DNA Microarrays (Gene Chips)
21 Construction and Screening of Human Antibody Libraries Using Phage Display Technology
22 Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology
23 Strategies for Gene Cloning, Expression, and Identification of Protein- Protein Interaction
24 Conditional Gene Knockout
25 How to Write a Research Manuscript for Publication in an English Journal
26 How to Protect Your Discovery and Invention
27 Determination of Transgene Copy Numbers and Practical Biocalculation

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Tags: William Wu, Helen H Zhang, Michael J Welsh, Peter B Kaufman, Gene Biotechnology