Fundamentals of Forensic DNA Typing 1st Edition by John M Butler ISBN 0123749999 9780123749994
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ISBN 10: 0123749999
ISBN 13: 9780123749994
Author: John M Butler
John Butler, bestselling author of Forensic DNA Typing, now applies his expertise on the subject of DNA analysis into an introductory textbook. Fundamentals of Forensic DNA Typing walks students step-by-step through the DNA analysis process beginning with collection of evidence at a crime scene to the statistical interpretation of the results. Also included are brief discussions of such news worthy topics as victim identification from the September 11, 2001 attacks, the identification of the remains of the Romanovs, the last Russian Royal family, and the O.J. Simpson case. New applications, such as genetic genealogy and tracing domestic pet hairs to perpetrators, are also detailed. With its clear and understandable style and extensive list of online ancillaries and study aids, this textbook will make the subject accessible to students in forensic science courses worldwide.
- Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect
- Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes– “Data, Notes & Applications” sections throughout
- Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key
Fundamentals of Forensic DNA Typing 1st Table of contents:
CHAPTER 1 Overview and History of DNA Typing
OVERVIEW OF THE CRIMINAL JUSTICE SYSTEM
A BRIEF HISTORY AND OVERVIEW OF FORENSIC DNA ANALYSIS
STEPS IN DNA SAMPLE PROCESSING
DNA ANALYSIS INVOLVES COMPARISONS
THE LABORATORY REPORT
COMPARISONS TO COMPUTER TECHNOLOGY
READING LIST AND INTERNET RESOURCES
CHAPTER 2 Basics of DNA Biology and Genetics
BASIC DNA PRINCIPLES
POPULATION VARIATION
INTRODUCTORY GENETIC PRINCIPLES
READING LIST AND INTERNET RESOURCES
CHAPTER 3 Historical Methods
A COMPARISON OF DNA TYPING METHODS
THE PRE-DNA YEARS (1900–1985)
THE FIRST DECADE OF DNA TESTING (1985–1995)
RFLP-BASED DNA TESTING
EARLY PCR-BASED DNA TESTING
THE SECOND DECADE OF DNA TESTING (1995–2005)
MITOCHONDRIAL DNA SEQUENCING
ADVANTAGES AND LIMITATIONS OF DNA TYPING METHODS
READING LIST AND INTERNET RESOURCES
CHAPTER 4 Sample Collection, Storage, and Characterization
SAMPLE COLLECTION
SAMPLE STORAGE
SAMPLE CHARACTERIZATION
READING LIST AND INTERNET RESOURCES
CHAPTER 5 DNA Extraction
DNA EXTRACTION
SOLID-PHASE DNA EXTRACTION METHODS
DIFFERENTIAL EXTRACTION
PCR INHIBITORS AND DNA DEGRADATION
READING LIST AND INTERNET RESOURCES
CHAPTER 6 DNA Quantitation
WHY QUANTIFY DNA?
DNA QUANTITIES USED
DNA QUANTITATION METHODS
COMPARISON OF METHODS
READING LIST AND INTERNET RESOURCES
CHAPTER 7 DNA Amplification (The Polymerase Chain Reaction)
POLYMERASE CHAIN REACTION (PCR) PROCESS
MULTIPLEX PCR
PCR INHIBITION
PRECAUTIONS AGAINST CONTAMINATION
READING LIST AND INTERNET RESOURCES
CHAPTER 8 Short Tandem Repeat Markers
GENETIC MARKERS AND REPEATED DNA SEQUENCES
CORE STR MARKERS
COMMERCIALLY AVAILABLE STR KITS
GENDER IDENTIFICATION WITH AMELOGENIN
STRBASE: A HELPFUL SOURCE OF INFORMATION ON STRS
READING LIST AND INTERNET RESOURCES
CHAPTER 9 Fundamentals of DNA Separation and Detection
DNA SEPARATION
DNA DETECTION
INSTRUMENT PLATFORMS FOR DNA SEPARATION AND DETECTION
READING LIST AND INTERNET RESOURCES
CHAPTER 10 STR Genotyping and Data Interpretation
STEPS IN STR GENOTYPING AND DATA INTERPRETATION
FACTORS AFFECTING GENOTYPING RESULTS
EXTRA PEAKS OBSERVED IN THE DATA
DEVELOPING AN INTERPRETATION STRATEGY
READING LIST AND INTERNET RESOURCES
CHAPTER 11 Statistical Interpretation: Evaluating the Strength of Forensic DNA Evidence
POPULATION DATA
GENETIC FORMULAS
METHODS FOR STATING THE RARITY OF A DNA PROFILE
FREQUENCY ESTIMATE CALCULATIONS
LIKELIHOOD RATIO
SOURCE ATTRIBUTION
ADDITIONAL STATISTICAL CALCULATIONS
READING LIST AND INTERNET RESOURCES
CHAPTER 12 DNA Databases
VALUE OF DNA DATABASES
ASPECTS OF A NATIONAL DNA DATABASE
NATIONAL DNA DATABASES AROUND THE WORLD
THE U.S. NATIONAL DNA DATABASE
DNA DATABASE LAWS IN THE UNITED STATES
ISSUES AND CONCERNS WITH DNA DATABASES
WHAT IF THERE ARE NO HITS AFTER A DATABASE SEARCH?
READING LIST AND INTERNET RESOURCES
CHAPTER 13 Quality Assurance
IMPORTANCE OF QUALITY ASSURANCE
LEVELS OF QA/QC
VALIDATION
OTHER AIDS TO QUALITY ASSURANCE
AUTOMATION
READING LIST AND INTERNET RESOURCES
CHAPTER 14 Forensic Challenges: Degraded DNA, Mixtures, and LCN
UNIQUE NATURE OF FORENSIC SAMPLES
DEGRADED DNA
MIXTURES
LOW COPY NUMBER DNA TESTING
READING LIST AND INTERNET RESOURCES
CHAPTER 15 Additional Loci and Nonhuman DNA Testing
BEYOND THE CORE GENETIC MARKERS
ADDITIONAL STR LOCI
SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS)
NONHUMAN DNA TESTING
READING LIST AND INTERNET RESOURCES
CHAPTER 16 Lineage Markers: Y Chromosome and mtDNA Testing
LINEAGE MARKERS
Y-CHROMOSOME DNA ANALYSIS
MITOCHONDRIAL DNA ANALYSIS
USING THE X CHROMOSOME IN IDENTITY TESTING
READING LIST AND INTERNET RESOURCES
CHAPTER 17 Applications of DNA Typing
PARENTAGE TESTING
DISASTER VICTIM IDENTIFICATION (DVI)
MISSING PERSONS INVESTIGATIONS
OTHER USES FOR STRs AND DNA TYPING METHODS
GENETIC GENEALOGY
READING LIST AND INTERNET RESOURCES
CHAPTER 18 Future Trends
ADDITIONAL GENETIC MARKERS
NEW TECHNOLOGIES
EXPANDED USES FOR DNA TESTING
READING LIST AND INTERNET RESOURCES
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