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Epiblast Stem Cells Methods and Protocols 1st Edition by Daniel C Factor, Fadi J Najm, Paul J Tesar, Ramiro Alberio ISBN 1627036288 9781627036283

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Epiblast Stem Cells Methods and Protocols 1st Edition Daniel C. Factor Digital Instant Download

Author(s): Daniel C. Factor, Fadi J. Najm, Paul J. Tesar (auth.), Ramiro Alberio (eds.)
ISBN(s): 9781627036283, 1627036288
Edition: 1
File Details: PDF, 6.26 MB
Year: 2013
Language: english
SKU: EB-4333188 Category: Tags: , , ,

Epiblast Stem Cells Methods and Protocols 1st Edition by Daniel C Factor, Fadi J Najm, Paul J Tesar, Ramiro Alberio – Ebook PDF Instant Download/Delivery: 1627036288, 9781627036283
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Product details:

ISBN 10: 1627036288 
ISBN 13: 9781627036283
Author: Daniel C Factor, Fadi J Najm, Paul J Tesar, Ramiro Alberio

This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast.

Epiblast Stem Cells Methods and Protocols 1st Table of contents:

chapter 1 – derivation of epiblast stem cells
sources and isolation techniques
culture conditions and media
quality control and validation

chapter 2 – maintenance and culture
feeder-dependent vs. feeder-free systems
passaging and expansion
avoiding differentiation and senescence

chapter 3 – characterization and analysis
molecular markers
pluripotency assays
genetic and epigenetic profiling

chapter 4 – differentiation protocols
directed differentiation strategies
lineage-specific induction
functional assays of differentiated cells

chapter 5 – genetic manipulation
transfection and viral methods
CRISPR/Cas9 and genome editing
reporter lines and functional studies

chapter 6 – high-throughput applications
screening and assay development
drug testing and toxicity studies
omics approaches

chapter 7 – ethical and regulatory considerations
guidelines for stem cell research
consent and sourcing of materials
biosafety and compliance

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Tags: Daniel C Factor, Fadi J Najm, Paul J Tesar, Protocols