Embryonic Stem Cell Protocols 3rd Edition by Kursad Turksen ISBN 9781493929535 1493929534

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ISBN 10: 1493929534
ISBN 13: 9781493929535
Author: Kursad Turksen

This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickly, thus the kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved, which this book reflects. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and fully updated, __Embryonic Stem Cell Protocols, Third Edition__ serves as an ideal reference for researchers investigating this rich area of study.

Embryonic Stem Cell Protocols 3rd Edition Table of contents:

Part I: Introduction to Embryonic Stem Cells (ESCs)

1. Introduction to Stem Cell Biology
   1.1. Definition and Types of Stem Cells
   1.2. Historical Overview of ESC Research
   1.3. Basic Characteristics of Embryonic Stem Cells
   1.4. The Potential of ESCs in Regenerative Medicine

2. Ethical Considerations in ESC Research
   2.1. Ethical and Moral Issues Surrounding ESCs
   2.2. Legislative and Regulatory Frameworks
   2.3. Public Perception and Scientific Responsibility

3. The Origin and Isolation of Embryonic Stem Cells
   3.1. Embryonic Development and the Inner Cell Mass
   3.2. Techniques for Isolating ESCs from Mouse and Human Embryos
   3.3. Culture Conditions for ESCs

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Part II: Culturing and Expanding ESCs

4. Culturing Embryonic Stem Cells
   4.1. Basic Media and Supplements for ESC Culture
   4.2. Monolayer vs. Three-Dimensional Culture
   4.3. Maintaining Pluripotency in Culture
   4.4. Role of Feeder Cells in ESC Cultures

5. Cryopreservation of Embryonic Stem Cells
   5.1. Freezing and Thawing Protocols
   5.2. Preventing Loss of Pluripotency during Cryopreservation
   5.3. Storage Conditions and Long-Term Viability

6. Quality Control and Authentication of ESC Lines
   6.1. Genomic Stability and Pluripotency Markers
   6.2. karyotyping and SNP Analysis
   6.3. Epigenetic Profiling and Reproducibility of ESC Lines

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Part III: Differentiation Protocols

7. Induction of Differentiation in ESCs
   7.1. Methods for Directing ESC Differentiation
   7.2. Differentiation into Ectoderm, Mesoderm, and Endoderm Lineages
   7.3. Embryoid Body Formation for Spontaneous Differentiation
   7.4. Protocols for Neural, Cardiac, and Hepatic Differentiation

8. Directed Differentiation for Specific Lineages
   8.1. Methods for Neural Differentiation
   8.2. Cardiac Differentiation and Applications
   8.3. Differentiation of ESCs into Pancreatic β-cells
   8.4. Other Specialized Differentiation Protocols

9. Gene Editing and Genetic Modification of ESCs
   9.1. CRISPR/Cas9-based Gene Editing in ESCs
   9.2. Stable Transfection and Viral Transduction Methods
   9.3. Application of Gene Editing for Disease Modeling and Therapeutics

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Part IV: Applications of ESC Research

10. Stem Cell-Based Disease Modeling
    10.1. Creating Disease Models Using ESCs
    10.2. ESCs in Modeling Neurological Disorders
    10.3. Cardiac and Cancer Models from ESCs
    10.4. In Vitro Models for Drug Screening

11. Regenerative Medicine and Tissue Engineering
    11.1. ESCs in Tissue Repair and Regeneration
    11.2. Stem Cell Therapy and Clinical Applications
    11.3. Organ Regeneration from ESC-Derived Tissues
    11.4. Challenges and Future Directions in Regenerative Medicine

12. ESCs in Transplantation Therapy
    12.1. Immunological Barriers and ESC Transplantation
    12.2. Immunoprivileged ESCs and Personalized Medicine
    12.3. ESCs in Clinical Trials and Therapeutic Applications

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Tags: Kursad Turksen, Embryonic Stem, Cell Protocols