Dictyostelium discoideum Protocols 2nd Edition by Christina Schilde, Pauline Schaap, Ludwig Eichinger, Francisco Rivero – Ebook PDF Instant Download/Delivery: 1627033025, 9781627033022
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ISBN 10: 1627033025
ISBN 13: 9781627033022
Author: Christina Schilde, Pauline Schaap, Ludwig Eichinger, Francisco Rivero
Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.
Dictyostelium discoideum Protocols 2nd Table of contents:
The Amoebozoa
Christina Schilde, Pauline Schaap
Pages 1-15
The Model Organism Dictyostelium discoideum
Salvatore Bozzaro
Pages 17-37
Comparative Genomics of the Dictyostelids
William F. Loomis
Pages 39-58
One Stop Shop for Everything Dictyostelium: dictyBase and the Dicty Stock Center in 2012
Petra Fey, Robert J. Dodson, Siddhartha Basu, Rex L. Chisholm
Pages 59-92
Fluorescent Reporters and Methods to Analyze Fluorescent Signals
Annette Müller-Taubenberger, Hellen C. Ishikawa-Ankerhold
Pages 93-112
Collection and Cultivation of Dictyostelids from the Wild
Tracy E. Douglas, Debra A. Brock, Boahemaa Adu-Oppong, David C. Queller, Joan E. Strassmann
Pages 113-124
Identification and Verification of microRNAs by High-Throughput Sequencing
Jimmie Hällman, Lotta Avesson, Johan Reimegård, Max Käller, Fredrik Söderbom
Pages 125-138
Transcriptional Profiling of Dictyostelium with RNA Sequencing
Edward Roshan Miranda, Gregor Rot, Marko Toplak, Balaji Santhanam, Tomaz Curk, Gad Shaulsky et al.
Pages 139-171
Analysis of Chromatin Organization by Deep Sequencing Technologies
James L. Platt, Nick A. Kent, Adrian J. Harwood, Alan R. Kimmel
Pages 173-183
Pharmacogenetics of Resistance to Cisplatin and Other Anticancer Drugs and the Role of Sphingolipid Metabolism
Stephen Alexander, William S. Swatson, Hannah Alexander
Pages 185-204
N-Glycomic and N-Glycoproteomic Studies in the Social Amoebae
Christa L. Feasley, Alba Hykollari, Katharina Paschinger, Iain B. H. Wilson, Christopher M. West
Pages 205-229
Measuring Cheating, Fitness, and Segregation in Dictyostelium discoideum
Neil J. Buttery, Jeff Smith, David C. Queller, Joan E. Strassmann
Pages 231-248
The Application of the Cre-loxP System for Generating Multiple Knock-out and Knock-in Targeted Loci
Jan Faix, Joern Linkner, Benjamin Nordholz, James L. Platt, Xin-Hua Liao, Alan R. Kimmel
Pages 249-267
Extrachromosomal Inducible Expression
Douwe M. Veltman, Peter J. M. Van Haastert
Pages 269-281
Isolation of Dictyostelium Nuclei for Light and Electron Microscopy
Petros Batsios, Otto Baumann, Ralph Gräf, Irene Meyer
Pages 283-294
Investigation of DNA Repair Pathway Activity
Anne-Marie C. Couto, Nicholas D. Lakin, Catherine J. Pears
Pages 295-310
Transcript Localization in Dictyostelium discoideum Cells by RNA FISH
Patrick Hofmann, Janis Kruse, Christian Hammann
Pages 311-323
Analysis of Mitochondrial Gene Expression
Jessica E. Accari, Sam Manna, Paul R. Fisher, Christian Barth
Pages 325-344
Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction
Sarah J. Annesley, Sergio Carilla-Latorre, Ricardo Escalante, Paul R. Fisher
Pages 345-366
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Tags: Christina Schilde, Pauline Schaap, Ludwig Eichinger, Francisco Rivero, Dictyostelium, Protocols



