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Cytometric Analysis of Cell Phenotype and Function 1st Edition by Desmond A McCarthy, Marion G Macey ISBN 0521660297 9780521660297

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Cytometric Analysis of Cell Phenotype and Function 1st Edition Desmond A. Mccarthy Digital Instant Download

Author(s): Desmond A. McCarthy, Marion G. Macey
ISBN(s): 9780521660297, 0521660297
Edition: 1
File Details: PDF, 16.35 MB
Year: 2001
Language: english
SKU: EB-1737934 Category: Tags: ,

Cytometric Analysis of Cell Phenotype and Function 1st Edition by Desmond A McCarthy, Marion G Macey – Ebook PDF Instant Download/Delivery: 0521660297, 9780521660297
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Product details:

ISBN 10: 0521660297 
ISBN 13: 9780521660297
Author: Desmond A McCarthy, Marion G Macey

Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings due to improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding, and apoptosis. The volume guides the reader through data interpretation, quality control procedures, pitfalls, and problems.

 Cytometric Analysis of Cell Phenotype and Function 1st Table of contents:

Section I: Fundamentals of Cytometry

  1. Introduction to Cytometry

    • Principles of Cytometry and Cellular Analysis
    • Overview of Cytometric Techniques: Flow Cytometry, Mass Cytometry, and Imaging Cytometry
    • Advantages and Limitations of Cytometric Approaches

  2. Instrumentation and Technology

    • Basic Components of Cytometers: Lasers, Detectors, and Electronics
    • Advances in Cytometric Technologies: From Flow to Mass Cytometry
    • Automation and High-Throughput Cytometric Platforms

  3. Fluorescence and Cell Markers

    • Principles of Fluorescence in Cytometry
    • Selection of Fluorochromes and Antibodies for Cell Phenotyping
    • Multiplexing and Panel Design for Comprehensive Analysis

Section II: Phenotypic Analysis of Cells

  1. Cell Surface Markers and Phenotype Characterization

    • Identification of Cell Populations Using Surface Markers
    • Immunophenotyping: Techniques and Applications
    • Standard Protocols for Cell Phenotyping in Immunology and Hematology

  2. Intracellular Phenotyping and Functional Analysis

    • Intracellular Staining for Cytokines, Transcription Factors, and Signaling Molecules
    • Detection of Cellular Activation and Inhibition Markers
    • Challenges in Intracellular Cytometric Assays

  3. Cell Cycle and Proliferation Analysis

    • Assessing Cell Cycle Progression Using Cytometric Tools
    • Proliferation Assays: From DNA Synthesis to Apoptosis
    • Quantification of Cell Cycle Arrest and Drug Effects

Section III: Functional Analysis of Cells

  1. Assessing Cell Viability and Apoptosis

    • Methods for Evaluating Cell Health and Viability
    • Apoptosis Detection: Caspase Activity, Annexin V, and Mitochondrial Integrity
    • Necrosis vs. Apoptosis: How Cytometry Differentiates Cell Death Pathways

  2. Cellular Activation and Cytokine Profiling

    • Functional Assays to Assess Immune Cell Activation
    • Cytokine Secretion and Intracellular Cytometry in Immune Function
    • Detecting T-Cell and B-Cell Responses in Health and Disease

  3. Phagocytosis and Cellular Effector Functions

    • Measurement of Phagocytic Activity in Macrophages and Neutrophils
    • T-Cell and NK-Cell Cytotoxicity Assays
    • Assessing Functional Impairments in Disease Models

Section IV: Advanced Cytometric Techniques and Data Analysis

  1. Mass Cytometry and Single-Cell Proteomics

    • Introduction to Mass Cytometry and Its Applications
    • Mass Cytometry for Profiling Complex Cellular Networks
    • Data Analysis and Interpretation in Mass Cytometry

  2. Imaging Cytometry and High-Content Analysis

    • Integration of Cytometry and Imaging Techniques
    • Quantification of Cellular Features Using High-Content Screening
    • Applications in Drug Discovery and Cancer Research

  3. Advanced Data Analysis and Bioinformatics

    • Introduction to Cytometric Data Analysis
    • Gating Strategies, Compensation, and Data Normalization
    • Advanced Bioinformatics Tools for Cytometry: Cluster Analysis, Dimensionality Reduction, and Machine Learning

Section V: Applications of Cytometric Analysis

  1. Immunology and Infectious Disease

    • Cytometric Approaches to Immune Profiling in Health and Disease
    • Applications in Infectious Disease Immunology: HIV, Malaria, and Tuberculosis
    • Cytometry in Vaccine Development and Immune Responses

  2. Cancer Research and Tumor Immunology

    • Cytometric Profiling of Tumor Microenvironments
    • Immune Checkpoint Analysis and Cancer Immunotherapy
    • Cytometric Approaches in Liquid Biopsy and Cancer Diagnostics

  3. Stem Cell and Regenerative Medicine

    • Stem Cell Phenotyping and Function in Regenerative Medicine
    • Cytometry in Stem Cell Differentiation and Characterization
    • Stem Cell Therapy and the Role of Cytometry in Clinical Trials

  4. Neuroimmunology and Neuroscience

    • Cytometric Approaches to Brain Immune Cell Profiling
    • Immune System Activation in Neurological Diseases
    • Cellular Dynamics in Neurodegenerative Disorders

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