Sale!

Cytokine Bioassays Methods and Protocols 1st Edition by Ivana Vancurova ISBN 1493909274 9781493909278

Original price was: $50.00.Current price is: $35.00.

Cytokine Bioassays Methods and Protocols 1st Edition Ivana Vancurova (Eds.) Digital Instant Download

Author(s): Ivana Vancurova (eds.)
ISBN(s): 9781493909278, 1493909274
Edition: 1
File Details: PDF, 8.37 MB
Year: 2014
Language: english
SKU: EB-4691430 Category: Tag:

Cytokine Bioassays Methods and Protocols 1st Edition by Ivana Vancurova – Ebook PDF Instant Download/Delivery: 1493909274, 9781493909278
Full download Cytokine Bioassays Methods and Protocols 1st Edition after payment

Product details:

ISBN 10: 1493909274 
ISBN 13: 9781493909278
Author: Ivana Vancurova

Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenges including their low abundance, the need to distinguish between active and latent cytokine forms, and the need to measure multiple cytokines in a single assay. This volume will provide a comprehensive collection of classic and cutting-edge methodologies that are currently used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. The chapters are divided into four main categories. The first group focuses on the immunodetection of released cytokines in tissue culture supernatants, plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELISPOT and the antibody-based proximity ligation. The second group will focus on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced cell degranulation assay, cell proliferation and differentiation, cytokine-induced cytokine production and the radioreceptor cytokine assay. The third group focuses on the analysis of intracellular cytokines by flow cytometry, western blotting and fluorescence and confocal microscopy. In addition, this category includes protocols for quantitative analysis of cytokine gene expression by real time RT-PCR and analysis of the cytokine promoter occupancy by chromatin immunoprecipitation. The fourth group focuses on the recently developed multiplex arrays that can measure multiplecytokines in the same sample at the same time. This group includes quantification of multiple cytokines using cytometric bead arrays, ELISPOT assays, proteomics cytokine evaluation, multiplexed proximity ligation assays for high-throughput cytokine analysis and finally, cytokine gene expression analysis by gene arrays. The protocols will be written by experienced basic and clinical researchers with hands-on knowledge of the described protocols. By covering a broad variety of methods used in cytokine detection and analysis, this book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.

Cytokine Bioassays Methods and Protocols 1st Table of contents:

The Challenge of Measuring Elusive Immune Markers by Enzyme-Linked Immuno-Spot (ELISPOT) Technique
Maria Faresjö
Pages 3-12
Analysis of the Released Nuclear Cytokine HMGB1 in Human Serum
Haichao Wang, Lin Zhao, Jianhua Li, Shu Zhu, Maggie Yeung
Pages 13-25
ELISA-Based Assay for IP-10 Detection from Filter Paper Samples
Camilla Heldbjerg Drabe, Thomas Blauenfeldt, Morten Ruhwald
Pages 27-37
Enhanced ELISA Based on Carboxymethylated Dextran Coatings
Benoît Liberelle, Charles Fortier, Gregory De Crescenzo
Pages 39-47
A Novel Three-Dimensional Biosensor Based on Aluminum Oxide: Application for Early-Stage Detection of Human Interleukin-10
Michael Lee, Abdoullatif Baraket, Nadia Zine, Miguel Zabala, Francesca Campabadal, Raffaele Caruso et al.
Pages 49-64
Quantification of Multiple Cytokines and Chemokines Using Cytometric Bead Arrays
Gemma Moncunill, Joseph J. Campo, Carlota Dobaño
Pages 65-86
A Useful Guide for Analysis of Immune Markers by Fluorochrome (Luminex) Technique
Maria Faresjö
Pages 87-96
Cytokine Bioassays
Front Matter
Pages 97-97
Download chapter PDF 
Control of Pro-inflammatory Cytokine Release from Human Monocytes with the Use of an Interleukin-10 Monoclonal Antibody
Hardik Patel, Dennis Davidson
Pages 99-106
Cytokine-Induced Neutrophil Chemotaxis Assay
Dennis Davidson, Hardik Patel
Pages 107-113
An In Vitro One-Dimensional Assay to Study Growth Factor-Regulated Tumor Cell–Macrophage Interaction
Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, Robert J. Eddy
Pages 115-123
Chemotactic Responses by Macrophages to a Directional Source of a Cytokine Delivered by a Micropipette
Michael Cammer, Dianne Cox
Pages 125-135
Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry
Lokesh Sharma, Wenjun Wu, Sanjay L. Dholakiya, Samir Gorasiya, Jiao Wu, Ravikumar Sitapara et al.
Pages 137-145
Assessment of Cytokine-Modulated Proteasome Activity
Christopher J. Kirk, Saul R. Powell, Edmund J. Miller
Pages 147-162
Evaluation of the Adverse Effect of Low Concentration of Cadmium on Interleukin-4 Induced Class Switch Recombination in Burkett’s Lymphoma Raji Cell Line
Vladimir Poltoratsky
Pages 163-171
A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-stimulated Macrophage Cell Lysates
Veronika Miskolci, Désirée Spiering, Dianne Cox, Louis Hodgson
Pages 173-184
Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylation Method
Mahmud Arif Pavel, Clarissa Lam, Parul Kashyap, Zahra Salehi-Najafabadi, Gurpreet Singh, Yong Yu
Pages 185-192
Detection of CXCR2 Cytokine Receptor Surface Expression Using Immunofluorescence
Clarissa Lam, Mahmud Arif Pavel, Parul Kashyap, Zahra Salehi-Najafabadi, Victoria Valentino, Yong Yu
Pages 193-200

People also search for Cytokine Bioassays Methods and Protocols 1st:

cytokine assay protocol
    
cytokine bioassay
    
bioassay-guided fractionation
    
bioassay pharmacology
    
bioassay system

Tags: Ivana Vancurova, Cytokine Bioassays, Protocols