Culture of Epithelial Cells Second Edition by Ian Freshney ISBN 9780471401216 0471401218
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Product details:
ISBN 10: 0471401218
ISBN 13: 9780471401216
Author: R. Ian Freshney
“…a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years.”
–Pharmaceutical Research
This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Table of contents:
Chapter 1. Introduction — R. Ian Freshney
Chapter 2. Cell Interaction and Epithelial Differentiation — Nicole Maas-Szabowski, Hans-Jürgen Stark, and Norbert E. Fusenig
Chapter 3. The Epidermis — E. Kenneth Parkinson and W. Andrew Yeudall
Chapter 4. Culture of Human Mammary Epithelial Cells — Martha R. Stampfer, Paul Yaswen, and Joyce Taylor-Papadimitriou
Chapter 5. Culture of Human Cervical Epithelial Cells — Margaret A. Stanley
Chapter 6. Human Prostatic Epithelial Cells — Donna M. Peehl
Chapter 7. Human Oral Epithelium — Roland G. Grafstrom
Chapter 8. Normal Human Bronchial Epithelial Cell Culture — John Wise and John F. Lechner
Chapter 9. Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells — Leland G. Dobbs and Robert F. Gonzalez
Chapter 10. Isolation and Culture of Intestinal Epithelial Cells — Catherine Booth and Julie A. O’Shea
Chapter 11. Isolation and Culture of Animal and Human Hepatocytes — Christiane Guguen-Guillouzo
Chapter 12. Culture of Human Urothelium — Jennifer Southgate, John R. W. Masters, and Ludwik K. Trejdosiewicz
Chapter 13. Other Epithelial Cells — R. Ian Freshney
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