Carbohydrate Biotechnology Protocols 1st Edition by Christopher Bucke 9781617370748 1617370746

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ISBN 10:1617370746 
ISBN 13:9781617370748
Author:Christopher Bucke
We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.

Carbohydrate Biotechnology Protocols 1st Table of contents:

Introduction to Carbohydrate Biotechnology

Production and Isolation of Xanthan Gum

Alginate from Azotobacter vinelandii

Production of Schizophyllan

Enzymatic Synthesis of Cellulose

Modification of Alginate Using Mannuronan C-5-Epimerases

Viscosity Control of Guar Polysaccharide Solutions by Treatment with Galactose Oxidase and Catalase Enzymes

The Production of Cyclodextrins Using CGTase from Bacillus macerans

Production of Microbial Glycolipids

Partial Enzymatic Hydrolysis of Starch to Maltodextrins on the Laboratory Scale

The Production of α(1→2)-Terminated Glucooligosaccharides

Enzymatic Production of Fructooligosaccharides from Sucrose

Enzymatic Production of Inulooligosaccharides from Inulin

One-Pot Enzymatic Synthesis of Sialyl T-Epitope

Hydrolysis of Hemicelluloses Using Combinations of Xylanases and Feruloyl Esterases

Enzymatic Depolymerization of Chitins and Chitosans

Synthesis of Homo- and Hetero-Oligosaccharides from Underivatized Sugars Using Glycosidases

Use of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE®) in the Elucidation of N-Linked Oligosaccharide Structures

Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides

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Christopher Bucke,Biotechnology,Protocols,Carbohydrate