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C elegans Methods and Applications 3rd Edition by Gal Haspel, Anne C Hart ISBN 1071621807 9781071621806

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C elegans Methods and Applications 3rd Edition Gal Haspel (Editor) Digital Instant Download

Author(s): Gal Haspel (editor), Anne C. Hart (editor)
ISBN(s): 9781071621806, 1071621807
Edition: 3
File Details: PDF, 8.63 MB
Year: 2022
Language: english
SKU: EB-42608094 Category: Tags: ,

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ISBN 10: 1071621807 
ISBN 13: 9781071621806
Author: Gal Haspel, Anne C Hart

This detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, Caenorhabditis elegans, a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, C. elegans: Methods and Applications, Third Edition offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms.

C elegans Methods and Applications 3rd Table of contents:

  1. Starting a C. elegans Research Laboratory: Practical Advice

  2. The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans

  3. Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose

  4. Genetic Methods for Cellular Manipulation in C. elegans

  5. Observing and Quantifying Fluorescent Reporters

  6. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior

  7. Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior

  8. Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR)

  9. ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy

  10. A Fusion-PCR Method for Expressing Genetic Tools in C. elegans

  11. Approaches for CRISPR/Cas9 Genome Editing in C. elegans

  12. Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans

  13. Mutation Mapping and Identification by Whole-Genome Sequencing

  14. Lipid Extraction and Analysis

  15. Isolating Caenorhabditis elegans from the Natural Habitat

  16. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans

  17. Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

  18. An Imaging System for Monitoring C. elegans Behavior and Aging

  19. Methods for Modulating and Measuring Neuromuscular Exertion in C. elegans

  20. Recording and Quantifying C. elegans Behavior

  21. Primer on Mathematical Modeling in C. elegans

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Tags: Gal Haspel, Anne C Hart, C elegans