Bacterial Transcriptional Control Methods and Protocols 1st Edition by Irina Artsimovitch, Thomas J Santangelo ISBN 1493923919 9781493923915

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Product details:

ISBN 10: 1493923919 
ISBN 13: 9781493923915
Author: Irina Artsimovitch, Thomas J Santangelo

This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.

Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future

Bacterial Transcriptional Control Methods and Protocols 1st Table of contents:

Part I: Overview and Fundamental Concepts

• Introduction to Bacterial Transcriptional Regulation

• Overview of Transcription Factors and Regulatory Networks

• Bacterial Promoters and Operator Sites

• Techniques for Studying Transcriptional Regulation

Part II: Molecular Methods for Analyzing Transcriptional Control

• DNA Footprinting and EMSA (Electrophoretic Mobility Shift Assay)

• Reporter Gene Fusions and Assays

• Chromatin Immunoprecipitation (ChIP) and ChIP-Seq

• RNA-seq and Transcriptome Analysis

• Quantitative PCR for Transcription Analysis

• Northern Blotting and RNA Stability Assays

Part III: Genetic and Biochemical Approaches

• Mutagenesis Techniques for Regulatory Gene Analysis

• Overexpression and Knockdown of Transcription Factors

• Use of CRISPR and Genome Editing in Bacterial Transcription

• Protein-DNA Interaction Assays

• In Vitro Transcription Systems

Part IV: Computational and Systems Biology Approaches

• Bioinformatic Tools for Promoter and Regulatory Sequence Analysis

• Modeling Transcriptional Networks

• Integration of Multi-Omics Data

Part V: Protocols and Experimental Procedures

• Detailed Step-by-Step Experimental Protocols

• Troubleshooting and Optimization Tips

• Case Studies and Examples of Bacterial Transcriptional Control Experiments

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Tags: Irina Artsimovitch, Thomas J Santangelo, Bacterial Transcriptional, Control Methods