Antibiotic Resistance Protocols 2nd Edition by Stephen H Gillespie, Timothy D McHugh ISBN 1603272798 9781603272797

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ISBN 10: 1603272798 
ISBN 13: 9781603272797
Author: Stephen H Gillespie, Timothy D McHugh

Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.

Antibiotic Resistance Protocols 2nd Table of contents:

Part I: Epidemiology and Population Genetics

1. The RM Test for Determining Methicillin-Resistant Staphylococcus aureus (MRSA) Lineages
   Jodi A. Lindsay and Julia M.-L. Sung

2. Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx
   Bambos M. Charalambous and Ndeky M. Oriyo

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Part II: Genomics and Gene Expression

3. Methods to Determine Antibiotic Resistance Gene Silencing
   Virve I. Enne and Peter M. Bennett

4. Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping
   Hee-Jeon Hong

5. Real-Time PCR Methods to Study Expression of Genes Related to Hypermutability
   Denise O’Sullivan

6. Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds
   Simon J. Waddell and Philip D. Butcher

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Part III: Fitness, Mutation, and Physiology

7. Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity
   Mirjana Macvanin and Diarmaid Hughes

8. Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium
   Diarmaid Hughes and Mirjana Macvanin

9. Methods to Determine Fitness in Bacteria
   Cassie F. Pope, Timothy D. McHugh, and Stephen H. Gillespie

10. Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis
    Joanna Bacon, Kim A. Hatch, and Jon Allnutt

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Part IV: Detection of Resistance

11. Identification of Efflux-Mediated Multidrug Resistance in Bacterial Clinical Isolates by Two Simple Methods
    Marta Martins, Isabel Couto, Miguel Viveiros, and Leonard Amaral

12. Evaluation of Efflux Activity of Bacteria by a Semi-Automated Fluorometric System
    Miguel Viveiros et al.

13. Measuring the Activity of Active Efflux in Gram-Negative Bacteria
    Mark A. Webber and Nick G. Coldham

14. Rapid Characterization of β-Lactamases by Multiplex PCR
    Neil Woodford

15. Rapid Methods for Testing Inhibitors of Mycobacterial Growth
    Dimitrios Evangelopoulos and Sanjib Bhakta

16. Use of Biofilm Model Systems to Study Antimicrobial Susceptibility
    Rosaleen Anderson and M. J. Zagnitko

17. Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®
    Stephen H. Gillespie et al.

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Tags: Stephen H Gillespie, Timothy D McHugh, Antibiotic Resistance